Construct: ORF ccsbBroad304_10822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012229.1_s304c1, BRDN0000409115
- Derived from:
- ccsbBroadEn_10822
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FHL3 (2275)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10822
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2275 | FHL3 | four and a half LIM domains 3 | NM_001243878.2 | 99.8% | 100% | 216G>A |
| 2 | human | 2275 | FHL3 | four and a half LIM domains 3 | XM_017000675.1 | 76.3% | 60.6% | (many diffs) |
| 3 | human | 2275 | FHL3 | four and a half LIM domains 3 | NM_004468.5 | 61.3% | 61.4% | 1_324del;540G>A |
| 4 | human | 2275 | FHL3 | four and a half LIM domains 3 | XM_024454099.1 | 61.3% | 61.4% | 1_324del;540G>A |
| 5 | human | 2275 | FHL3 | four and a half LIM domains 3 | XM_024454100.1 | 61.3% | 61.4% | 1_324del;540G>A |
| 6 | human | 2275 | FHL3 | four and a half LIM domains 3 | XM_024454101.1 | 61.3% | 61.4% | 1_324del;540G>A |
| 7 | mouse | 14201 | Fhl3 | four and a half LIM domains 3 | NM_010213.3 | 53.8% | 58.1% | (many diffs) |
| 8 | mouse | 14201 | Fhl3 | four and a half LIM domains 3 | XM_006502768.3 | 53.8% | 58.1% | (many diffs) |
| 9 | mouse | 14201 | Fhl3 | four and a half LIM domains 3 | XM_006502766.3 | 51.3% | 55.4% | (many diffs) |
| 10 | mouse | 14201 | Fhl3 | four and a half LIM domains 3 | XM_006502769.3 | 21.4% | 20.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgggtcccgg aagctggaat atggaggcca gacatggcat gagcactgct 121 tcctgtgcag tggctgtgaa cagccactgg gctcccgttc ttttgtgccc gacaagggtg 181 ctcactactg cgtgccctgc tatgagaaca agtttgctcc tcgctgcgcc cgctgcagca 241 agacgctgac acagggtgga gtgacatacc gtgatcagcc atggcatcga gaatgtctgg 301 tctgtaccgg atgccagacg cccctggcag ggcagcagtt cacctcccgg gatgaagatc 361 cctactgtgt ggcctgtttt ggagaactct ttgcacctaa gtgcagcagc tgcaagcgcc 421 ccatcgtagg actcggtgga ggcaagtatg tgtcctttga agaccgacac tggcaccaca 481 actgcttctc ctgcgcccgc tgctctacct ccctggtggg ccagggcttc gtaccggatg 541 gagaccaagt gctctgccag ggctgtagcc aggcagggcc ctacccaact ttcttgtaca 601 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 661 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta