Transcript: Human NM_004468.5

Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
FHL3 (2275)
Length:
1656
CDS:
179..1021

Additional Resources:

NCBI RefSeq record:
NM_004468.5
NBCI Gene record:
FHL3 (2275)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004468.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417871 TCGAGGGAGCTGTTCTATGAA pLKO_005 329 CDS 100% 5.625 7.875 N FHL3 n/a
2 TRCN0000430104 CACTAGCCGATGAACCCTTCA pLKO_005 399 CDS 100% 4.050 5.670 N FHL3 n/a
3 TRCN0000423035 ACTCGGTGGAGGCAAGTATGT pLKO_005 868 CDS 100% 4.950 3.960 N FHL3 n/a
4 TRCN0000152069 CCCTTAAATCTAGACTTCTCT pLKO.1 1361 3UTR 100% 3.000 2.400 N FHL3 n/a
5 TRCN0000113527 GTTCTATGAAGACCGCCATTT pLKO.1 340 CDS 100% 10.800 7.560 N Fhl3 n/a
6 TRCN0000113525 CCCTGCTATGAGAACAAGTTT pLKO.1 632 CDS 100% 5.625 3.938 N Fhl3 n/a
7 TRCN0000151840 CAAGTATGTGTCCTTTGAAGA pLKO.1 880 CDS 100% 4.950 3.465 N FHL3 n/a
8 TRCN0000152789 GAGAACTCTTTGCACCTAAGT pLKO.1 819 CDS 100% 4.950 3.465 N FHL3 n/a
9 TRCN0000423282 TGGAATATGGAGGCCAGACAT pLKO_005 522 CDS 100% 4.950 3.465 N FHL3 n/a
10 TRCN0000423052 TGGAGTGACATACCGTGATCA pLKO_005 694 CDS 100% 4.950 3.465 N FHL3 n/a
11 TRCN0000158247 CATCGAGAATGTCTGGTCTGT pLKO.1 722 CDS 100% 2.640 1.848 N FHL3 n/a
12 TRCN0000156834 GCAAGTACATCCAGACAGACA pLKO.1 228 CDS 100% 2.640 1.848 N FHL3 n/a
13 TRCN0000113529 CTGTTCTATGAAGACCGCCAT pLKO.1 338 CDS 100% 2.160 1.512 N Fhl3 n/a
14 TRCN0000154294 GCTATGACAATACCTTTGCCA pLKO.1 270 CDS 100% 0.750 0.525 N FHL3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004468.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00569 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00569 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469104 GCGCGAGTGAGTCCCCGGTAGCTC pLX_317 45.9% 100% 100% V5 n/a
4 ccsbBroadEn_10822 pDONR223 100% 61.3% 61.4% None 1_324del;540G>A n/a
5 ccsbBroad304_10822 pLX_304 0% 61.3% 61.4% V5 1_324del;540G>A n/a
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