Construct: ORF ccsbBroad304_10837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006430.1_s304c1, BRDN0000404443
- Derived from:
- ccsbBroadEn_10837
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GARS1 (2617)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10837
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2617 | GARS1 | glycyl-tRNA synthetase 1 | NM_002047.4 | 98.4% | 97.7% | 2196_2197insA;2217_2218ins35 |
| 2 | human | 2617 | GARS1 | glycyl-tRNA synthetase 1 | NM_001316772.1 | 91.2% | 90.5% | 0_1ins162;2034_2035insA;2055_2056ins35 |
| 3 | human | 2617 | GARS1 | glycyl-tRNA synthetase 1 | XM_006715686.2 | 82% | 81.3% | 0_1ins369;1827_1828insA;1848_1849ins35 |
| 4 | mouse | 353172 | Gars | glycyl-tRNA synthetase | NM_180678.3 | 85.4% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2319
- ORF length:
- 2253
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctctccgcgt ccagtgctgc ttagaggtgc tcgcgccgct ctgctgctgc 121 tgctgccgcc ccggctctta gcccgaccct cgctcctgct ccgccggtcc ctcagcgcgg 181 cctcctgccc cccgatctcc ttgcccgccg ccgcctcccg gagcagcatg gacggcgcgg 241 gggctgagga ggtgctggca cctctgaggc tagcagtgcg ccagcaggga gatcttgtgc 301 gaaaactcaa agaagataaa gcaccccaag tagacgtaga caaagcagtg gctgagctca 361 aagcccgcaa gagggttctg gaagcaaagg agctggcgtt acagcccaaa gatgatattg 421 tagaccgagc aaaaatggaa gataccctga agaggaggtt tttctatgat caagcttttg 481 ctatttatgg aggtgttagt ggtctgtatg actttgggcc agttggctgt gctttgaaga 541 acaatattat tcagacctgg aggcagcact ttatccaaga ggaacagatc ctggagatcg 601 attgcaccat gctcacccct gagccagttt taaagacctc tggccatgta gacaaatttg 661 ctgacttcat ggtgaaagac gtaaaaaatg gagaatgttt tcgtgctgac catctattaa 721 aagctcattt acagaaattg atgtctgata agaagtgttc tgtcgaaaag aaatcagaaa 781 tggaaagtgt tttggcccag cttgataact atggacagca agaacttgcg gatctttttg 841 tgaactataa tgtaaaatct cccattactg gaaatgatct atcccctcca gtgtctttta 901 acttaatgtt caagactttc attgggcctg gaggaaacat gcctgggtac ttgagaccag 961 aaactgcaca ggggattttc ttgaatttca aacgactttt ggagttcaac caaggaaagt 1021 tgccttttgc tgctgcccag attggaaatt cttttagaaa tgagatctcc cctcgatctg 1081 gactgatcag agtcagagaa ttcacaatgg cagaaattga gcactttgta gatcccagtg 1141 agaaagacca ccccaagttc cagaatgtgg cagaccttca cctttatttg tattcagcaa 1201 aagcccaggt cagcggacag tccgctcgga aaatgcgcct gggagatgct gttgaacagg 1261 gtgtgattaa taacacagta ttaggctatt tcattggccg catctacctc tacctcacga 1321 aggttggaat atctccagat aaactccgct tccggcagca catggagaat gagatggccc 1381 attatgcctg tgactgttgg gatgcagaat ccaaaacatc ctacggttgg attgagattg 1441 ttggatgtgc tgatcgttcc tgttatgacc tctcctgtca tgcacgagcc accaaagtcc 1501 cacttgtagc tgagaaacct ctgaaagaac ccaaaacagt caatgttgtt cagtttgaac 1561 ccagtaaggg agcaattggt aaggcatata agaaggatgc aaaactggtg atggagtatc 1621 ttgccatttg tgatgagtgc tacattacag aaatggagat gctgctgaat gagaaagggg 1681 aattcacaat tgaaactgaa gggaaaacat ttcagttaac aaaagacatg atcaatgtga 1741 agagattcca gaaaacacta tatgtggaag aagttgttcc gaatgtaatt gaaccttcct 1801 tcggcctggg taggatcatg tatacggtat ttgaacatac attccatgta cgagaaggag 1861 atgaacagag aacattcttc agtttccctg ctgtagttgc tccattcaaa tgttccgtcc 1921 tcccactgag ccaaaaccag gagttcatgc catttgtcaa ggaattatcg gaagccctga 1981 ccaggcatgg agtatctcac aaagtagacg attcctctgg gtcaatcgga aggcgctatg 2041 ccaggactga tgagattggc gtggcttttg gtgtcaccat tgactttgac acagtgaaca 2101 agacccccca cactgcaact ctgagggacc gtgactcaat gcggcagata agagcagaga 2161 tctctgagct gcccagcata gtccaagacc tagccaatgg caacatcaca tgggctgatg 2221 tggaggccag gtatcctctg tttgaagggc aagagactgg taaaaaaaga gacaatcgag 2281 gaatgaggac aattttgaca acttttgacc acttgcgcta cccaactttc ttgtacaaag 2341 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2401 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta