Construct: ORF ccsbBroad304_10913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001512.1_s304c1, BRDN0000409706
- Derived from:
- ccsbBroadEn_10913
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGL (3535)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10913
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 96.6% | 97.1% | (many diffs) |
| 2 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001178126.2 | 62.7% | 50.6% | (many diffs) |
| 3 | human | 3543 | IGLL1 | immunoglobulin lambda like ... | NM_020070.4 | 57.2% | 41.7% | (many diffs) |
| 4 | human | 3543 | IGLL1 | immunoglobulin lambda like ... | NM_001369906.1 | 57% | 43.8% | (many diffs) |
| 5 | human | 3543 | IGLL1 | immunoglobulin lambda like ... | NM_152855.3 | 24% | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaan 61 gccccatggc ctgctgcgcc caatggttgc accgcaaagc ggggacccag accctggagc 121 ctcagttgga agcagccgat ccagcctgcg gagcctgtgg ggcaggtcag cccaaggctg 181 ccccctcggt cactctgttc ccgccctcct ctgaggagct tcaagccaac aaggccacac 241 tggtgtgtct cataagtgac ttctacccgg gagccgtgac agtggcctgg aaggcagata 301 gcagccccgt caaggcggga gtggagacca ccacaccctc caaacaaagc aacaacaagt 361 acgcggccag cagctacctg agcctgacgc ctgagcagtg gaagtcccac agaagctaca 421 gctgccaggt cacgcatgaa gggagcaccg tggagaagac agtggcccct acagaatgtt 481 catacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 541 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 601 tctggatta