Construct: ORF ccsbBroad304_10930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001270.1_s304c1, BRDN0000410729
- Derived from:
- ccsbBroadEn_10930
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNA2 (3737)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10930
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3737 | KCNA2 | potassium voltage-gated cha... | NM_001204269.2 | 99.9% | 100% | 219A>G |
| 2 | human | 3737 | KCNA2 | potassium voltage-gated cha... | NM_004974.4 | 66.9% | 60.9% | (many diffs) |
| 3 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_011541396.2 | 66.9% | 60.9% | (many diffs) |
| 4 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_011541397.2 | 66.9% | 60.9% | (many diffs) |
| 5 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_011541398.2 | 66.9% | 60.9% | (many diffs) |
| 6 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_011541399.2 | 66.9% | 60.9% | (many diffs) |
| 7 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_011541400.2 | 66.9% | 60.9% | (many diffs) |
| 8 | human | 3737 | KCNA2 | potassium voltage-gated cha... | XM_017001213.1 | 66.9% | 60.9% | (many diffs) |
| 9 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | NM_008417.5 | 63% | 61.6% | (many diffs) |
| 10 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501048.3 | 63% | 61.6% | (many diffs) |
| 11 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501049.3 | 63% | 61.6% | (many diffs) |
| 12 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501050.3 | 63% | 61.6% | (many diffs) |
| 13 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501051.3 | 63% | 61.6% | (many diffs) |
| 14 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501052.3 | 63% | 61.6% | (many diffs) |
| 15 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501053.3 | 63% | 61.6% | (many diffs) |
| 16 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501054.3 | 63% | 61.6% | (many diffs) |
| 17 | mouse | 16490 | Kcna2 | potassium voltage-gated cha... | XM_006501055.3 | 63% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac agtggccacc ggagacccag cagacgaggc tgctgccctc cctgggcacc 121 cacaggacac ctatgaccca gaggcagacc acgagtgctg tgagagggtg gtgatcaaca 181 tctcagggct gcggtttgag acccagctaa agaccttagc ccagtttcca gagaccctct 241 taggggaccc aaagaaacga atgaggtact ttgaccccct ccggaatgag tactttttcg 301 atcggaaccg ccctagcttt gatgccattt tgtactacta ccagtcaggg ggccgattga 361 ggcgacctgt gaatgtgccc ttagatatat tctctgaaga aattcggttt tatgagctgg 421 gagaagaagc gatggagatg tttcgggaag atgaaggcta catcaaggag gaagagcgtc 481 ctctgcctga aaatgagttt cagagacaag tgtggcttct ctttgaatac ccagagagct 541 cagggcctgc caggattata gctattgtgt ctgtcatggt gattctgatc tcaattgtca 601 gcttctgtct ggaaacattg cccatcttcc gggatgagaa tgaagacatg catggtagtg 661 gggtgacctt ccacacctat tccaacagca ccatcgggta ccagcagtcc acttccttca 721 cagacccttt cttcattgta gagacactct gcatcatctg gttctccttt gaattcttgg 781 tgaggttctt tgcctgtccc agcaaagccg gcttcttcac caacatcatg aacatcattg 841 acattgtggc catcatcccc tacttcatca ccctggggac agagttggct gagaagccag 901 aggacgctca gcaaggccag caggccatgt cactggccat cctccgtgtc atccggttgg 961 aacgcagacc tctgcaaagc cagaagagta agcggggaag gcagcatctg aacacctcac 1021 atgactgcac cttaggaatt aacctagtcg cgggcatgac tgtacagtgg accagggcat 1081 ctggtcctga tgacaggcag acaccagctg taactacatt gcacaggatg tattgcccaa 1141 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1201 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta