Construct: ORF ccsbBroad304_10985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003230.1_s304c1, BRDN0000411485
- Derived from:
- ccsbBroadEn_10985
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R12B (4660)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10985
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | NM_001167858.1 | 36.6% | 36.5% | 424_425delTAinsAG;427_1158del |
| 2 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | NM_001167857.1 | 27.4% | 27.3% | 424_425delTAinsAG;427_1545del |
| 3 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_005245206.4 | 25.2% | 25.1% | 424_425delTAinsAG;427_1680del |
| 4 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001351.1 | 25.2% | 25.1% | 424_425delTAinsAG;427_1680del |
| 5 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737202.1 | 17.8% | 1_150del;574_575delTAinsAG;577_2370del | |
| 6 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737199.1 | 14.4% | 1_150del;574_575delTAinsAG;577_2929del | |
| 7 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001348.1 | 14.4% | 14.3% | 424_425delTAinsAG;427_2943del |
| 8 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | NM_002481.4 | 14.3% | 14.3% | 424_425delTAinsAG;427_2946del |
| 9 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001347.1 | 14.1% | 14.1% | 424_425delTAinsAG;427_2991del |
| 10 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001346.1 | 14.1% | 14.1% | 424_425delTAinsAG;427_2994del |
| 11 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737196.2 | 13.8% | 1_150del;574_575delTAinsAG;577_3053del | |
| 12 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_002956646.1 | 13.7% | 1_150del;574_575delTAinsAG;577_3073del | |
| 13 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001345.1 | 13.5% | 13.5% | 424_425delTAinsAG;427_3126del |
| 14 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | NM_001331029.2 | 13.5% | 13.5% | 424_425delTAinsAG;427_3129del |
| 15 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737195.1 | 13.4% | 1_150del;574_575delTAinsAG;577_3159del | |
| 16 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001343.1 | 13.3% | 13.3% | 424_425delTAinsAG;427_3174del |
| 17 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XM_017001342.1 | 13.3% | 13.3% | 424_425delTAinsAG;427_3177del |
| 18 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737201.2 | 4.4% | 1_150del;574_575delTAinsAG;577_9566del | |
| 19 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737198.1 | 3.7% | 1_150del;574_575delTAinsAG;577_11252del | |
| 20 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737200.1 | 3.7% | 1_150del;574_575delTAinsAG;577_11252del | |
| 21 | human | 4660 | PPP1R12B | protein phosphatase 1 regul... | XR_001737197.1 | 3.7% | 1_150del;574_575delTAinsAG;577_11435del | |
| 22 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XR_387177.3 | 16% | (many diffs) | |
| 23 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XM_017321278.1 | 14.3% | 14.7% | (many diffs) |
| 24 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XM_006529710.2 | 13.5% | 13.9% | (many diffs) |
| 25 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | NM_001081307.1 | 13.3% | 13.7% | (many diffs) |
| 26 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XM_006529709.3 | 13.3% | 13.7% | (many diffs) |
| 27 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XM_017321277.1 | 12.7% | 13.1% | (many diffs) |
| 28 | mouse | 329251 | Ppp1r12b | protein phosphatase 1, regu... | XM_006529707.3 | 12.5% | 12.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaactggag cacctaggag ggaagcgggc agagtcggcg cgaatgcggc 121 gggcagagca gcttcggcgc tggcggggct cgctgacaga gcaggagcct gcggagcgac 181 gaggcgcggg gcggcagccg ctgaccaggc gcgggagccc cagggtccgc ttcgaggacg 241 gtgctgtctt tctggccgcc tgctctagcg gggacaccga cgaggtgaga aagcttctgg 301 caagaggtgc tgatatcaac acggtcaacg tggacggctt gacagccctg caccaggcat 361 gtattgatga aaatttggac atggtgaagt ttctggtgga gaacagagcc aatgtaaacc 421 agcaagacaa cgagggctgg acaccccttc atgcagcagc ttcctgtggc tatctcaaca 481 tagcagagag ttgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 541 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 601 caatcaacct ctggatta