Transcript: Human NM_001167858.1

Homo sapiens protein phosphatase 1 regulatory subunit 12B (PPP1R12B), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
PPP1R12B (4660)
Length:
1751
CDS:
151..1311

Additional Resources:

NCBI RefSeq record:
NM_001167858.1
NBCI Gene record:
PPP1R12B (4660)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001167858.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231498 TGACATGGATATTCGAAATAA pLKO_005 972 CDS 100% 15.000 10.500 N PPP1R12B n/a
2 TRCN0000231499 CCAATTCCAGGGAACCTATAA pLKO_005 1401 3UTR 100% 13.200 9.240 N PPP1R12B n/a
3 TRCN0000231497 ACGGAGCCAGTGTAGGTATTG pLKO_005 587 CDS 100% 10.800 7.560 N PPP1R12B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001167858.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10985 pDONR223 100% 36.6% 36.5% None 424_425delTAinsAG;427_1158del n/a
2 ccsbBroad304_10985 pLX_304 0% 36.6% 36.5% V5 424_425delTAinsAG;427_1158del n/a
3 TRCN0000472803 TTCCCCCCTTCGGTTTGAAAACGC pLX_317 80.9% 36.6% 36.5% V5 424_425delTAinsAG;427_1158del n/a
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