Construct: ORF ccsbBroad304_11000

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012789.1_s304c1, BRDN0000398906
Derived from:
ccsbBroadEn_11000
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NONO (4841)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11000
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4841 NONO non-POU domain containing o... NM_001145410.1 89% 89% 1_126del
2 human 4841 NONO non-POU domain containing o... NM_001145408.2 72.1% 72.1% 1_393del
3 human 4841 NONO non-POU domain containing o... NM_001145409.2 72.1% 72.1% 1_393del
4 human 4841 NONO non-POU domain containing o... NM_007363.5 72.1% 72.1% 1_393del
5 mouse 53610 Nono non-POU-domain-containing, ... NM_001252518.1 66.3% 71.4% (many diffs)
6 mouse 53610 Nono non-POU-domain-containing, ... NM_023144.2 66.3% 71.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1086
ORF length:
1020
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc actccgtgga aagcagctgc gtgtgcgctt tgcctgccat agtgcatccc
121ttacagttcg aaaccttcct cagtatgtgt ccaacgaact gctggaagaa gccttttctg
181tgtttggcca ggtagagagg gctgtagtca ttgtggatga tcgaggaagg ccctcaggaa
241aaggcattgt tgagttctca gggaagccag ctgctcggaa agctctggac agatgcagtg
301aaggctcctt cctgctaacc acatttcctc gtcctgtgac tgtggagccc atggaccagt
361tagatgatga agagggactt ccagagaagc tggttataaa aaaccagcaa tttcacaagg
421aacgagagca gccacccaga tttgcacagc ctggctcctt tgagtatgaa tatgccatgc
481gctggaaggc actcattgag atggagaagc agcagcagga ccaagtggac cgcaacatca
541aggaggctcg tgagaagctg gagatggaga tggaagctgc acgccatgag caccaggtca
601tgctaatgag acaggatttg atgaggcgcc aagaagaact tcggaggatg gaagagctgc
661acaaccaaga ggtgcaaaaa cgaaagcaac tggagctcag gcaggaggaa gagcgcaggc
721gccgtgaaga agagatgcgg cggcagcaag aagaaatgat gcggcgacag caggaaggat
781tcaagggaac cttccctgat gcgagagagc aggagattcg gatgggtcag atggctatgg
841gaggtgctat gggcataaac aacagaggtg ccatgccccc tgctcctgtg ccagctggta
901ccccagctcc tccaggacct gccactatga tgccggatgg aactttggga ttgaccccac
961caacaactga acgctttggt caggctgcta caatggaagg aattggggca attggtggaa
1021ctcctcctgc attcaaccgt gcagctcctg gagctgaatt tgccccaaac aaacgtcgcc
1081gatactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1141gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1201acctctggat ta

Download FASTA (ORF) (Full)