Construct: ORF ccsbBroad304_11006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011421.1_s304c1, BRDN0000409633
- Derived from:
- ccsbBroadEn_11006
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NVL (4931)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11006
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4931 | NVL | nuclear VCP like | NM_001243146.1 | 93.2% | 93.2% | 0_1ins57;90G>A;312_392del |
| 2 | human | 4931 | NVL | nuclear VCP like | XM_017001386.2 | 88.7% | 88.3% | (many diffs) |
| 3 | human | 4931 | NVL | nuclear VCP like | NM_206840.2 | 87.3% | 86.9% | (many diffs) |
| 4 | human | 4931 | NVL | nuclear VCP like | XM_024447367.1 | 87.3% | 86.9% | (many diffs) |
| 5 | human | 4931 | NVL | nuclear VCP like | NM_001243147.1 | 86.1% | 86.1% | 1_318del;465G>A |
| 6 | human | 4931 | NVL | nuclear VCP like | XM_011544199.1 | 85.6% | 85.2% | (many diffs) |
| 7 | human | 4931 | NVL | nuclear VCP like | XM_011544200.1 | 85.6% | 85.2% | (many diffs) |
| 8 | human | 4931 | NVL | nuclear VCP like | XM_011544198.2 | 84.2% | 83.9% | (many diffs) |
| 9 | human | 4931 | NVL | nuclear VCP like | XM_017001383.1 | 80.6% | 80.2% | (many diffs) |
| 10 | human | 4931 | NVL | nuclear VCP like | XM_017001381.1 | 78% | 77.7% | (many diffs) |
| 11 | human | 4931 | NVL | nuclear VCP like | XM_017001382.1 | 78% | 77.7% | (many diffs) |
| 12 | human | 4931 | NVL | nuclear VCP like | NM_002533.4 | 76.5% | 76.1% | (many diffs) |
| 13 | human | 4931 | NVL | nuclear VCP like | XM_011544196.1 | 74.1% | 73.8% | (many diffs) |
| 14 | human | 4931 | NVL | nuclear VCP like | XM_017001379.1 | 73.6% | 73.2% | (many diffs) |
| 15 | human | 4931 | NVL | nuclear VCP like | XM_017001378.1 | 71.4% | 71.1% | (many diffs) |
| 16 | human | 4931 | NVL | nuclear VCP like | XM_017001380.2 | 67.9% | 66.9% | (many diffs) |
| 17 | human | 4931 | NVL | nuclear VCP like | XR_001737205.1 | 66.4% | (many diffs) | |
| 18 | human | 4931 | NVL | nuclear VCP like | XR_001737204.1 | 65.1% | (many diffs) | |
| 19 | human | 4931 | NVL | nuclear VCP like | XM_017001384.1 | 57.4% | 57% | (many diffs) |
| 20 | human | 4931 | NVL | nuclear VCP like | XM_017001385.2 | 53.8% | 53.4% | (many diffs) |
| 21 | mouse | 67459 | Nvl | nuclear VCP-like | XM_011238857.2 | 78.3% | 80.3% | (many diffs) |
| 22 | mouse | 67459 | Nvl | nuclear VCP-like | XM_006496964.3 | 77.1% | 79% | (many diffs) |
| 23 | mouse | 67459 | Nvl | nuclear VCP-like | XM_011238856.2 | 69.4% | 71.1% | (many diffs) |
| 24 | mouse | 67459 | Nvl | nuclear VCP-like | NM_026171.2 | 67.6% | 69.3% | (many diffs) |
| 25 | mouse | 67459 | Nvl | nuclear VCP-like | XM_006496965.3 | 56.5% | 56.8% | (many diffs) |
| 26 | mouse | 67459 | Nvl | nuclear VCP-like | XR_001785531.1 | 32.5% | (many diffs) | |
| 27 | mouse | 67459 | Nvl | nuclear VCP-like | XR_373614.3 | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2043
- ORF length:
- 1977
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agactaccca gatccacagg attcaaaaga ttcttctctt ttggagagtg 121 atatgaaacg gaaaggcaag ctaaagaata aaggaagcaa aaggaagaaa gaagatcttc 181 aggaagtaga tggagaaatt gaagctgtcc tacaaaagaa agctaaagcc agggggttag 241 aattccagat ctccaacgtg aagtttgaag atgtgggagg caatgatatg acattaaaag 301 aggtctgcaa gatgctcata cacatgcgtc acccggaggt gtaccaccac ctgggcgtcg 361 tgccccctcg tggagttctc cttcatggac caccaggctg tgggaagaca ttacttgcac 421 atgcaattgc tggggaactt gacctgccaa ttttgaaagt ggctgctcca gagattgtgt 481 ctggagtatc cggagagtct gagcagaagc tgagagaact atttgagcaa gctgtgtcaa 541 atgcaccatg tatcattttc attgatgaaa ttgatgctat tacccccaaa agagaagtgg 601 cttcaaaaga tatggaacga agaattgtag cccaactcct aacctgcatg gatgatctga 661 ataatgtggc tgctacagcc cgggtcctag ttattggagc tactaatcga ccagactcgt 721 tagaccctgc tttgagacgt gcgggaaggt tcgaccgaga aatatgccta ggtatcccag 781 atgaagcatc cagggaaaga atacttcaaa cattgtgcag aaaactgagg cttcctcaag 841 cttttgattt ctgtcactta gcacacctaa ctccaggctt tgttggtgct gatctcatgg 901 cactgtgccg agaggcagca atgtgtgcag tcaatagagt cttaatgaag ctacaggaac 961 agcagaagaa aaatcctgaa atggaagatt tgccatctaa aggagtccag gaggaaaggc 1021 tgggaactga gcccacttct gaaacacagg atgaattaca aaggctgctg gggttgctaa 1081 gagaccaaga tcccctctca gaggagcaga tgcaaggact gtgcattgaa ctgaatgatt 1141 tcattgttgc tctatcctca gtccaaccct ctgccaaaag ggaaggcttt gtcactgtcc 1201 ctaatgtgac atgggcagat attggtgccc tggaagacat tagagaggag ctcaccatgg 1261 caatattggc accagtacgc aacccagacc agttcaaagc tcttggattg gtgactccag 1321 ctggggtcct ccttgctggt cctcctggct gtgggaagac tctgctggcg aaggctgttg 1381 caaatgagtc cggactaaat tttatatctg tcaagggccc cgaattacta aacatgtatg 1441 ttggtgagag tgaacgtgct gtgcgacaag tttttcaacg agccaagaac tcagcaccct 1501 gtgtgatatt ctttgatgaa gtggatgctt tatgtcctcg aagatcagac cgagagacag 1561 gggcaagtgt ccgagtggtg aatcagctac ttacagagat ggatggtctg gaagcacgcc 1621 agcaggtttt tattatggca gccactaaca ggccagatat aattgaccct gcaatcctgc 1681 gcccgggccg cctggacaaa acactgtttg tgggtttacc gccccctgca gatcgccttg 1741 ccatcttaaa aactatcaca aaaaatggta ccaaaccacc actggatgca gatgtaaatt 1801 tggaagcaat tgctggtgac cttcgctgtg attgctatac gggcgcagat ctctctgctt 1861 tggtacgaga agcttctatc tgtgccctga gacaggaaat ggcaagacag aagagtggaa 1921 atgaaaaagg tgaactcaag gttagtcata agcattttga agaagctttc aagaaagtaa 1981 gatcatctat atcaaaaaag gatcaaatca tgtatgaacg tttgcaggag tccctcagcc 2041 ggtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2101 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2161 tctggatta