Construct: ORF ccsbBroad304_11025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016251.1_s304c1, BRDN0000406844
- Derived from:
- ccsbBroadEn_11025
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PER1 (5187)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11025
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5187 | PER1 | period circadian regulator 1 | XM_024450803.1 | 70.1% | 67.6% | 1630_1735del;1823_2445del |
| 2 | human | 5187 | PER1 | period circadian regulator 1 | XM_005256690.1 | 46.5% | 44.7% | 1630_1735del;1823_3690del |
| 3 | human | 5187 | PER1 | period circadian regulator 1 | NM_002616.3 | 44.3% | 42.7% | 1630_1735del;1823_3870del |
| 4 | human | 5187 | PER1 | period circadian regulator 1 | XM_005256689.2 | 44.3% | 42.7% | 1630_1735del;1823_3870del |
| 5 | mouse | 18626 | Per1 | period circadian clock 1 | XM_006532481.3 | 39.5% | 41.5% | (many diffs) |
| 6 | mouse | 18626 | Per1 | period circadian clock 1 | NM_001159367.1 | 39.4% | 41.5% | (many diffs) |
| 7 | mouse | 18626 | Per1 | period circadian clock 1 | NM_011065.4 | 39.4% | 41.5% | (many diffs) |
| 8 | mouse | 18626 | Per1 | period circadian clock 1 | XM_006532480.3 | 39.4% | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tggcccccta gaaggggctg atgggggagg ggaccccagg cctggggaat 121 cattttgtcc tgggggcgtc ccatcccctg ggcccccaca gcaccggcct tgcccaggcc 181 ccagcctggc cgatgacacc gatgccaaca gcaatggttc aagtggcaat gagtccaacg 241 ggcatgagtc tagaggcgca tctcagcgga gctcacacag ctcctcctca ggcaacggca 301 aggactcagc cctgctggag accactgaga gcagcaagag cacaaactct cagagcccat 361 ccccacccag cagttccatt gcctacagcc tcctgagtgc cagctcagag caggacaacc 421 cgtccaccag tggctgcagc agtgaacagt cagcccgggc aaggactcag aaggaactca 481 tgacagcact tcgagagctc aagcttcgac tgccgccaga gcgccggggc aagggccgct 541 ctgggaccct ggccacgctg cagtacgcac tggcctgtgt caagcaggtg caggccaacc 601 aggaatacta ccagcagtgg agcctggagg agggcgagcc ttgctccatg gacatgtcca 661 cctataccct ggaggagctg gagcacatca cgtctgagta cacacttcag aaccaggata 721 ccttctcagt ggctgtctcc ttcctgacgg gccgaatcgt ctacatttcg gagcaggcag 781 ccgtcctgct gcgttgcaag cgggacgtgt tccggggtac ccgcttctct gagctcctgg 841 ctccccagga tgtgggagtc ttctatggtt ccactgctcc atctcgcctg cccacctggg 901 gcacaggggc ctcagcaggt tcaggcctca gggactttac ccaggagaag tccgtcttct 961 gccgtatcag aggaggtcct gaccgggatc cagggcctcg gtaccagcca ttccgcctaa 1021 ccccgtatgt gaccaagatc cgggtctcag atggggcccc tgcacagccg tgctgcctgc 1081 tgattgcaga gcgcatccat tcgggttacg aagctccccg gataccccct gacaagagga 1141 ttttcactac gcggcacaca cccagctgcc tcttccagga tgtggatgaa agggctgccc 1201 ccctgctggg ctacctgccc caggacctcc tgggggcccc agtgctcctg ttcctgcatc 1261 ctgaggaccg acccctcatg ctggctatcc acaagaagat tctgcagttg gcgggccagc 1321 cctttgacca ctcccctatc cgcttctgtg cccgcaacgg ggagtatgtc accatggaca 1381 ccagctgggc tggctttgtg cacccctgga gccgcaaggt agccttcgtg ttgggccgcc 1441 acaaagtacg cacggccccc ctgaatgagg acgtgttcac tcccccggcc cccagcccag 1501 ctccctccct ggacactgat atccaggagc tgtcagagca gatccaccgg ctgctgctgc 1561 agcccgtcca cagccccagc cccacgggac tctgtggagt cggcgccgtg acatccccag 1621 gccctctcca cagccctggg tcctccagtg atagcaacgg gggtgatgca gaggggcctg 1681 ggcctcctgc gccactacag gcacgttcaa ggccaaggcc cttccctgcc aatccccaga 1741 cccagagctg gaggcgggtt ctgctcccgt ccaggcCCCA CCACccaact atcttgtaca 1801 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1861 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta