Construct: ORF ccsbBroad304_11091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001982.1_s304c1, BRDN0000411048
- Derived from:
- ccsbBroadEn_11091
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM4 (5936)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11091
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5936 | RBM4 | RNA binding motif protein 4 | NM_001198844.2 | 86.8% | 79.7% | (many diffs) |
| 2 | human | 5936 | RBM4 | RNA binding motif protein 4 | NM_001198843.1 | 79.7% | 78.5% | (many diffs) |
| 3 | human | 83759 | RBM4B | RNA binding motif protein 4B | NM_001286135.2 | 76.4% | 77.9% | (many diffs) |
| 4 | human | 83759 | RBM4B | RNA binding motif protein 4B | NM_031492.4 | 43.1% | 39.8% | (many diffs) |
| 5 | human | 83759 | RBM4B | RNA binding motif protein 4B | XM_011545297.3 | 43.1% | 39.8% | (many diffs) |
| 6 | human | 83759 | RBM4B | RNA binding motif protein 4B | XM_017018402.2 | 43.1% | 39.8% | (many diffs) |
| 7 | human | 5936 | RBM4 | RNA binding motif protein 4 | NM_002896.3 | 42.9% | 40.1% | (many diffs) |
| 8 | human | 83759 | RBM4B | RNA binding motif protein 4B | XR_247214.3 | 23.1% | (many diffs) | |
| 9 | human | 83759 | RBM4B | RNA binding motif protein 4B | XR_247213.3 | 22.2% | (many diffs) | |
| 10 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XM_006531827.3 | 78.1% | 79% | (many diffs) |
| 11 | mouse | 19653 | Rbm4 | RNA binding motif protein 4 | NM_001290124.1 | 73.7% | 77.4% | (many diffs) |
| 12 | mouse | 19653 | Rbm4 | RNA binding motif protein 4 | NM_001290125.1 | 73.7% | 77.4% | (many diffs) |
| 13 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | NM_025717.3 | 41.7% | 40% | (many diffs) |
| 14 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XM_006531823.3 | 41.7% | 40% | (many diffs) |
| 15 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XM_006531824.3 | 41.7% | 40% | (many diffs) |
| 16 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XM_006531825.3 | 41.7% | 40% | (many diffs) |
| 17 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XM_006531826.2 | 41.7% | 40% | (many diffs) |
| 18 | mouse | 19653 | Rbm4 | RNA binding motif protein 4 | NM_001290122.1 | 40.5% | 39.8% | (many diffs) |
| 19 | mouse | 19653 | Rbm4 | RNA binding motif protein 4 | NM_001290123.1 | 40.5% | 39.8% | (many diffs) |
| 20 | mouse | 19653 | Rbm4 | RNA binding motif protein 4 | NM_009032.3 | 40.5% | 39.8% | (many diffs) |
| 21 | mouse | 102902673 | Gm21992 | predicted gene 21992 | NM_001290127.1 | 30.8% | 30.1% | (many diffs) |
| 22 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XR_001782610.1 | 8% | (many diffs) | |
| 23 | mouse | 66704 | Rbm4b | RNA binding motif protein 4B | XR_001782611.1 | 5.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gaagctgttc atcggaaacc tgccccggga ggctacagag caggagattc 121 gctcactctt cgagcagtat gggaaggtgc tggaatgtga catcattaag aattacggct 181 ttgtgcacat agaagacaag acggcagctg aggatgccat acgcaacctg caccattaca 241 agcttcatgg ggtgaacatc aacgtggaag ccagcaagaa taagagcaaa acctcaacaa 301 agttgcatgt gggcaacatc agtcccacct gcaccaataa ggagcttcga gccaagtttg 361 aggagtatgg tccggtcatc gaatgtgaca tcgtgaaaga ttatgccttc gtacacatgg 421 agcgggcaga ggatgcagtg gaggccatca ggggccttga taacacagag tttcaaggtg 481 aaccaccctc tttgggtaga gggctgaaca caaggctgtg tgcagaaaat ggttggataa 541 gtaaaaggag aggtttggta aagataacag ctgttggctg gctggtgatg aagaaatacc 601 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 661 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 721 tta