Transcript: Mouse NM_001290124.1

Mus musculus RNA binding motif protein 4 (Rbm4), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Rbm4 (19653)
Length:
1912
CDS:
206..637

Additional Resources:

NCBI RefSeq record:
NM_001290124.1
NBCI Gene record:
Rbm4 (19653)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001290124.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263390 CATGATGCACTTCACTATTTA pLKO_005 1511 3UTR 100% 15.000 7.500 Y Rbm4 n/a
2 TRCN0000158726 GCTGGAATGTGACATCATTAA pLKO.1 289 CDS 100% 13.200 6.600 Y RBM4 n/a
3 TRCN0000103964 CCCAGTCATCGAATGTGACAT pLKO.1 511 CDS 100% 4.950 2.475 Y Rbm4 n/a
4 TRCN0000103960 CCTTGTTAAGTTCTTTCTCTT pLKO.1 746 3UTR 100% 4.950 2.475 Y Rbm4 n/a
5 TRCN0000103724 GCCAGCAAGAATAAGAGCAAA pLKO.1 410 CDS 100% 4.950 2.475 Y Rbm4b n/a
6 TRCN0000161617 GCCAGCAAGAATAAGAGCAAA pLKO.1 410 CDS 100% 4.950 2.475 Y RBM4B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001290124.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15562 pDONR223 0% 92.5% 98.6% None (many diffs) n/a
2 ccsbBroad304_15562 pLX_304 0% 92.5% 98.6% V5 (many diffs) n/a
3 TRCN0000479076 GACCCGACTCACAATTGGTGACGT pLX_317 84.4% 92.5% 98.6% V5 (many diffs) n/a
4 ccsbBroadEn_11091 pDONR223 100% 73.7% 77.4% None (many diffs) n/a
5 ccsbBroad304_11091 pLX_304 0% 73.7% 77.4% V5 (many diffs) n/a
6 TRCN0000471664 CTCAACATACTGAACCTTCAGTAA pLX_317 68.7% 73.7% 77.4% V5 (many diffs) n/a
7 ccsbBroadEn_09114 pDONR223 100% 37.4% 38.9% None (many diffs) n/a
8 ccsbBroad304_09114 pLX_304 0% 37.4% 38.9% V5 (many diffs) n/a
9 TRCN0000466951 CTTCCGCGACGGGCGACCCGGAAC pLX_317 15.6% 37.4% 38.9% V5 (many diffs) n/a
10 ccsbBroadEn_01382 pDONR223 100% 35.9% 37.9% None (many diffs) n/a
11 ccsbBroad304_01382 pLX_304 0% 35.9% 37.9% V5 (many diffs) n/a
12 TRCN0000467520 CATTGTCTACCGGTTTAACTCAAT pLX_317 39.8% 35.9% 37.9% V5 (many diffs) n/a
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