Construct: ORF ccsbBroad304_11104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008410.1_s304c1, BRDN0000402609
- Derived from:
- ccsbBroadEn_11104
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL5 (6125)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11104
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6125 | RPL5 | ribosomal protein L5 | NM_000969.5 | 34% | 32.9% | 292_361del;374_891del |
| 2 | human | 6125 | RPL5 | ribosomal protein L5 | NR_146333.1 | 29.5% | 1_129del;433_1024del | |
| 3 | mouse | 100503670 | Rpl5 | ribosomal protein L5 | XM_006534684.2 | 30.5% | 32.7% | (many diffs) |
| 4 | mouse | 100503670 | Rpl5 | ribosomal protein L5 | NM_016980.2 | 30.4% | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gtttgttaaa gttgttaaga ataaggccta ctttaagaga taccaagtga 121 aatttagaag acgacgagag ggtaaaactg attattatgc tcggaaacgc ttggtgatac 181 aagataaaaa taaatacaac acacccaaat acaggatgat agttcgtgtg acaaacagag 241 atatcatttg tcagattgct tatgcccgta tagaggggga tatgatagtc tgcgcagcgt 301 atgcacacga actgccaaaa tatggtgtga aggttggcct gacaaattat gctgcagcca 361 agtggaggtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 421 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 481 tcaacctctg gatta