Construct: ORF ccsbBroad304_11105
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016941.1_s304c1, BRDN0000410825
- Derived from:
- ccsbBroadEn_11105
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL12 (6136)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11105
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6136 | RPL12 | ribosomal protein L12 | NM_000976.4 | 80% | 80% | 111_209del |
| 2 | mouse | 269261 | Rpl12 | ribosomal protein L12 | NM_009076.3 | 71.3% | 79.3% | (many diffs) |
| 3 | mouse | 102635048 | LOC102635048 | 60S ribosomal protein L12 p... | XR_880074.1 | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccgaagttc gaccccaacg agatcaaagt cgtatacctg aggtgcaccg 121 gaggtgaagt cggtgccact tctgccctgg cccccaagat cggccccctg ggtctgattg 181 aggtggtgcc ttctgcctct gccctgatca tcaaagccct caaggaacca ccaagagaca 241 gaaagaaaca gaaaaacatt aaacacagtg ggaatatcac ttttgatgag attgtcaaca 301 ttgctcgaca gatgcggcac cgatccttag ccagagaact ctctggaacc attaaagaga 361 tcctggggac tgcccagtca gtgggctgta atgttgatgg ccgccatcct catgacatca 421 tcgatgacat caacagtggt gctgtggaat gcccagccag ttacccaact ttcttgtaca 481 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 541 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta