Construct: ORF ccsbBroad304_11132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014096.1_s304c1, BRDN0000406492
- Derived from:
- ccsbBroadEn_11132
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST3GAL4 (6484)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11132
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001254757.2 | 100% | 100% | |
| 2 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001254758.1 | 100% | 100% | |
| 3 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001348399.2 | 100% | 100% | |
| 4 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | XM_024448658.1 | 100% | 100% | |
| 5 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | XM_024448659.1 | 100% | 100% | |
| 6 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | XM_024448660.1 | 100% | 100% | |
| 7 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001254759.2 | 99.1% | 98.1% | (many diffs) |
| 8 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001348400.2 | 98.7% | 98.4% | 89_90insGTACATCGAGCT |
| 9 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_006278.3 | 98.7% | 98.4% | 89_90insGTACATCGAGCT |
| 10 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001348396.2 | 94% | 94% | 1_63del |
| 11 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001348397.2 | 94% | 94% | 1_63del |
| 12 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NM_001348398.2 | 79.1% | 73.2% | 89_90insGTACATCGAGCT;756_757ins82;906_1050del |
| 13 | human | 6484 | ST3GAL4 | ST3 beta-galactoside alpha-... | NR_145671.2 | 50.3% | 1_217del;986_1160del;1392_1985del | |
| 14 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | NM_009178.4 | 86.4% | 89.4% | (many diffs) |
| 15 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_011242433.1 | 86.4% | 89.4% | (many diffs) |
| 16 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_011242432.2 | 81.3% | 84.1% | (many diffs) |
| 17 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510111.3 | 78.8% | 81.6% | (many diffs) |
| 18 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510112.3 | 78.8% | 81.6% | (many diffs) |
| 19 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510113.3 | 78.8% | 81.6% | (many diffs) |
| 20 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_011242429.2 | 78.8% | 81.6% | (many diffs) |
| 21 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510110.3 | 78.4% | 81.1% | (many diffs) |
| 22 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510109.3 | 77.8% | 80.5% | (many diffs) |
| 23 | mouse | 20443 | St3gal4 | ST3 beta-galactoside alpha-... | XM_006510108.2 | 77.1% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt cagcaagtcc cgctggaagc tcctggccat gttggctctg gtcctggtcg 121 tcatggtgtg gtattccatc tcccgggaag acaggtacat cgagcttttt tattttccca 181 tcccagagaa gaaggagccg tgcctccagg gtgaggcaga gagcaaggcc tctaagctct 241 ttggcaacta ctcccgggat cagcccatct tcctgcggct tgaggattat ttctgggtca 301 agacgccatc tgcttacgag ctgccctatg ggaccaaggg gagtgaggat ctgctcctcc 361 gggtgctagc catcaccagc tcctccatcc ccaagaacat ccagagcctc aggtgccgcc 421 gctgtgtggt cgtggggaac gggcaccggc tgcggaacag ctcactggga gatgccatca 481 acaagtacga tgtggtcatc agattgaaca atgccccagt ggctggctat gagggtgacg 541 tgggctccaa gaccaccatg cgtctcttct accctgaatc tgcccacttc gaccccaaag 601 tagaaaacaa cccagacaca ctcctcgtcc tggtagcttt caaggcaatg gacttccact 661 ggattgagac catcctgagt gataagaagc gggtgcgaaa gggtttctgg aaacagcctc 721 ccctcatctg ggatgtcaat cctaaacaga ttcggattct caaccccttc ttcatggaga 781 ttgcagctga caaactgctg agcctgccaa tgcaacagcc acggaagatt aagcagaagc 841 ccaccacggg cctgttggcc atcacgctgg ccctccacct ctgtgacttg gtgcacattg 901 ccggctttgg ctacccagac gcctacaaca agaagcagac cattcactac tatgagcaga 961 tcacgctcaa gtccatggcg gggtcaggcc ataatgtctc ccaagaggcc ctggccatta 1021 agcggatgct ggagatggga gctatcaaga acctcacgtc cttctgccca actttcttgt 1081 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1141 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a