Transcript: Human NM_001348400.2

Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4), transcript variant 9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ST3GAL4 (6484)
Length:
1798
CDS:
218..1207

Additional Resources:

NCBI RefSeq record:
NM_001348400.2
NBCI Gene record:
ST3GAL4 (6484)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001348400.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005578 CCTGCGGCTTGAGGATTATTT pLKO.1 412 CDS 100% 15.000 21.000 N ST3GAL4 n/a
2 TRCN0000297059 CCTGCGGCTTGAGGATTATTT pLKO_005 412 CDS 100% 15.000 21.000 N ST3GAL4 n/a
3 TRCN0000005574 CCAGCACCAAGAGATTATTTA pLKO.1 1470 3UTR 100% 15.000 12.000 N ST3GAL4 n/a
4 TRCN0000005576 CCTGGTAGCTTTCAAGGCAAT pLKO.1 769 CDS 100% 4.050 2.835 N ST3GAL4 n/a
5 TRCN0000277891 CCTGGTAGCTTTCAAGGCAAT pLKO_005 769 CDS 100% 4.050 2.835 N ST3GAL4 n/a
6 TRCN0000005577 GCCACGGAAGATTAAGCAGAA pLKO.1 958 CDS 100% 4.050 2.835 N ST3GAL4 n/a
7 TRCN0000297123 GCCACGGAAGATTAAGCAGAA pLKO_005 958 CDS 100% 4.050 2.835 N ST3GAL4 n/a
8 TRCN0000005575 GCAATGGACTTCCACTGGATT pLKO.1 785 CDS 100% 4.950 2.970 N ST3GAL4 n/a
9 TRCN0000277939 GCAATGGACTTCCACTGGATT pLKO_005 785 CDS 100% 4.950 2.970 N ST3GAL4 n/a
10 TRCN0000018827 CCACTGGATTGAGACCATCTT pLKO.1 796 CDS 100% 4.950 3.465 N St3gal4 n/a
11 TRCN0000278752 CCACTGGATTGAGACCATCTT pLKO_005 796 CDS 100% 4.950 3.465 N St3gal4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348400.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11132 pDONR223 100% 98.7% 98.4% None 89_90insGTACATCGAGCT n/a
2 ccsbBroad304_11132 pLX_304 0% 98.7% 98.4% V5 89_90insGTACATCGAGCT n/a
3 TRCN0000468712 TGGTCCGGTGATTACACGGAGTCC pLX_317 34.5% 98.7% 98.4% V5 89_90insGTACATCGAGCT n/a
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