Construct: ORF ccsbBroad304_11136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000247.1_s304c1, BRDN0000402125
- Derived from:
- ccsbBroadEn_11136
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A11 (6538)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11136
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | NM_001317406.2 | 100% | 100% | |
| 2 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | XM_011534033.2 | 54.5% | 54.5% | 625_1143del |
| 3 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | NM_014229.3 | 32.9% | 32.9% | 625_1896del |
| 4 | mouse | 243616 | Slc6a11 | solute carrier family 6 (ne... | NM_172890.3 | 28.4% | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ggcggagaag gcgctgcccc tgggcaatgg gaaggctgct gaggaggcgc 121 gggagtccga ggcgccgggt ggcggctgca gcagcggggg cgcggcgccc gcgcgccacc 181 cgcgcgtcaa gcgcgacaag gcggtccacg agcgcggcca ctggaacaac aaggtggagt 241 tcgtgctgag cgtggccggg gagatcattg ggctgggcaa cgtgtggcgc ttcccctacc 301 tgtgctacaa gaacggagga ggggcattcc tgattcccta cgtggtgttt tttatttgct 361 gtggaattcc tgtttttttc ctggagacag ctctggggca gttcacaagt gaaggtggca 421 ttacgtgttg gaggaaagtt tgccctttat ttgaaggcat tggctatgca acacaggtga 481 ttgaggccca tctgaatgtg tactacatca tcatcctggc atgggccatt ttttacctga 541 gcaactgctt cactactgag ctaccctggg ctacctgtgg gcatgagtgg aacacagaga 601 attgtgtgga gttccagaaa ctgaatgtga gcaactacag ccatgtgtct ctgcagaatg 661 ccacctcccc tgtcatggag ttttgggagn ncccaacttt cttgtacaaa gtggttggta 721 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 781 ggtggcgcgt taagtcgaca atcaacctct ggatta