Construct: ORF ccsbBroad304_11145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012144.1_s304c1, BRDN0000410815
- Derived from:
- ccsbBroadEn_11145
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMARCA2 (6595)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11145
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_001289398.2 | 47.9% | 47.9% | 1_387del |
| 2 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_001289399.1 | 43.1% | 43.1% | 1_471del |
| 3 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_001289400.1 | 42.8% | 42.8% | 1_477del |
| 4 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_001289397.2 | 7.8% | 7.8% | 1_4185del |
| 5 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_139045.4 | 7.5% | 7.5% | 1_4359del |
| 6 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_001289396.1 | 7.4% | 7.4% | 1_4413del |
| 7 | human | 6595 | SMARCA2 | SWI/SNF related, matrix ass... | NM_003070.5 | 7.4% | 7.4% | 1_4413del |
| 8 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | NM_026003.2 | 44.2% | 46.6% | (many diffs) |
| 9 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527291.2 | 44.2% | 46.6% | (many diffs) |
| 10 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_017318276.1 | 44.2% | 46.6% | (many diffs) |
| 11 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_017318277.1 | 44.2% | 46.6% | (many diffs) |
| 12 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_017318278.1 | 44.2% | 46.6% | (many diffs) |
| 13 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | NM_001347439.1 | 37.8% | 39.8% | (many diffs) |
| 14 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527288.2 | 37.8% | 39.8% | (many diffs) |
| 15 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527289.2 | 37.8% | 39.8% | (many diffs) |
| 16 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527286.1 | 6.8% | 7.2% | (many diffs) |
| 17 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | NM_011416.2 | 6.6% | 6.9% | (many diffs) |
| 18 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527285.2 | 6.6% | 6.9% | (many diffs) |
| 19 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527281.3 | 6.5% | 6.8% | (many diffs) |
| 20 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527282.3 | 6.5% | 6.8% | (many diffs) |
| 21 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527283.3 | 6.5% | 6.8% | (many diffs) |
| 22 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XM_006527284.2 | 6.5% | 6.8% | (many diffs) |
| 23 | mouse | 67155 | Smarca2 | SWI/SNF related, matrix ass... | XR_386502.2 | 5.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tctctgtcac aacgctcaga cgttcaacct ggagggatcc cagatctatg 121 aagactccat cgtcttacag tcagtgttta agagtgcccg gcagaaaatt gccaaagagg 181 aagagagtga ggatgaaagc aatgaagagg aggaagagga agatgaagaa gagtcagagt 241 ccgaggcaaa atcagtcaag gtgaaaatta agctcaataa aaaagatgac aaaggccggg 301 acaaagggaa aggcaagaaa aggccaaatc gaggaaaagc caaacctgta gtgagcgatt 361 ttgacagcga tgaggagcag gatgaacgtg aacagtcaga aggaagtggg acggatgatg 421 agtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta