Construct: ORF ccsbBroad304_11157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017156.1_s304c1, BRDN0000461635
- Derived from:
- ccsbBroadEn_11157
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRP68 (6730)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11157
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6730 | SRP68 | signal recognition particle 68 | NM_014230.4 | 95% | 95% | 765_857del |
| 2 | human | 6730 | SRP68 | signal recognition particle 68 | NM_001260502.1 | 88.9% | 88.9% | 249_250ins114;651_743del |
| 3 | human | 6730 | SRP68 | signal recognition particle 68 | NR_048541.1 | 59% | (many diffs) | |
| 4 | human | 6730 | SRP68 | signal recognition particle 68 | NM_001260503.1 | 48.3% | 48.3% | 0_1ins924 |
| 5 | mouse | 217337 | Srp68 | signal recognition particle 68 | NM_146032.3 | 83.3% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1854
- ORF length:
- 1788
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgagaag caggtcccag gcggcggcgg cggcggcggc agtggcggcg 121 gcggtggcag tggcggcggc ggtagcggcg gtggacgtgg tgccggaggg gaagaaaata 181 aagaaaacga acgcccttcg gccggatcga aggcaaacaa agaatttggg gatagcctga 241 gtttggagat tcttcagatt attaaggaat cccagcagca gcatggttta cggcatggag 301 attttcagag gtacaggggc tactgttccc gtagacaaag acgtcttcga aaaacactca 361 acttcaagat gggtaacaga cacaaattca cagggaagaa agtgactgaa gagcttctga 421 ccgataatag atacttgctt ctggttctga tggatgctga aagagcctgg agctacgcca 481 tgcagctgaa acaggaagcc aacactgaac cccgaaaacg gtttcacttg ttatctcgcc 541 tacgcaaagc cgtgaagcat gcagaggaat tggaacgctt gtgtgagagc aatcgcgtgg 601 atgccaagac caaattagag gctcaggctt acacagctta cctctcagga atgctacgtt 661 ttgaacatca agaatggaaa gctgccattg aggcttttaa caaatgcaaa actatctatg 721 agaagctagc cagtgctttc acagaggagc aggctgtgct gtataaccaa cgtgtggaag 781 agatttcacc caacatccgc tattgtgcat ataatattgg ggaccagtca gccaaacagg 841 cagctaccat gagtgaagtg gagtggagag ggagaacggt tccagtgaag attgacaaag 901 tgcgcatttt cttattagga ctggctgata acgaagcagc tattgtccag gctgaaagcg 961 aagaaactaa ggagcgcctg tttgaatcaa tgctcagcga gtgtcgggac gccatccagg 1021 tggttcggga ggagctcaag ccagatcaga aacagagaga ttatatcctt gaaggagagc 1081 cagggaaggt gtctaatctt caatacttgc atagctacct gacttacatc aagctatcaa 1141 cggcaatcaa gcgtaatgag aacatggcca aaggtctgca gagggctctg ctgcagcagc 1201 agccagagga tgacagcaag cgctcacccc ggccccagga cctgatccga ctctatgaca 1261 tcatcttaca gaatctggtg gaattgctcc agcttcctgg tttagaggaa gacaaagcct 1321 tccagaaaga gataggcctc aagactctgg tgttcaaagc ttacaggtgt tttttcattg 1381 ctcagtccta tgtgctggtg aagaagtgga gcgaagccct tgtcctgtat gacagagtcc 1441 tgaaatatgc aaatgaagta aattctgatg ctggcgcctt caagaacagc ctaaaggacc 1501 tgcctgatgt gcaagagctc atcactcaag tgcggtcaga gaagtgctcc ctgcaggccg 1561 cagccatcct tgatgcaaac gacgctcatc aaacagagac ctcctcctcc caagtcaagg 1621 acaataagcc tctggttgaa cggtttgaga cattctgcct ggacccttcc cttgtcacca 1681 agcaagccaa ccttgtgcac ttcccaccag gcttccagcc cattccctgc aagcctttgt 1741 tctttgacct ggccctcaac catgtggctt tcccacccct tgaggacaag ttggaacaga 1801 agaccaagag tggcctcact ggatacatca agggcatctt tggattcagg agctacccaa 1861 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1921 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta