Construct: ORF ccsbBroad304_11163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008226.1_s304c1, BRDN0000402495
- Derived from:
- ccsbBroadEn_11163
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK4 (6789)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11163
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147975.2 | 12.5% | 1_57del;175_935del | |
| 2 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_017028033.2 | 10% | 10% | 118_1164del |
| 3 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_011529020.2 | 8.5% | 7.1% | (many diffs) |
| 4 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_001352385.2 | 8.4% | 8.4% | 118_1386del |
| 5 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_006282.5 | 8% | 8% | 118_1461del |
| 6 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260531.3 | 7.2% | 6% | (many diffs) |
| 7 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260530.2 | 6.8% | 5.7% | (many diffs) |
| 8 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147974.2 | 6.1% | 1_57del;174_1891delinsG | |
| 9 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499964.3 | 10.3% | 11.4% | (many diffs) |
| 10 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499963.3 | 8.1% | 9% | (many diffs) |
| 11 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | NM_021420.3 | 7.2% | 8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 183
- ORF length:
- 117
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gacggtacag ctgaggaacc cgccgcgccg gcagctgaaa aagttggatg 121 aagatagttt aaccaaacaa ccagaagaag tatttgatgt cttagagaaa cttggagaag 181 ggtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 241 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 301 tctggatta