Construct: ORF ccsbBroad304_11177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014646.1_s304c1, BRDN0000407368
- Derived from:
- ccsbBroadEn_11177
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCFC2 (6936)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11177
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | NM_001201335.2 | 99.8% | 99.5% | 181G>A |
| 2 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XM_011533075.2 | 49.1% | 48.8% | (many diffs) |
| 3 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XM_017004787.2 | 49.1% | 48.8% | (many diffs) |
| 4 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XR_002959327.1 | 28.7% | (many diffs) | |
| 5 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XM_011533074.3 | 26.9% | 19.1% | (many diffs) |
| 6 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | NM_003203.5 | 26.7% | 26.5% | (many diffs) |
| 7 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XM_005264520.4 | 26.4% | 26.2% | (many diffs) |
| 8 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XR_426995.3 | 24% | (many diffs) | |
| 9 | human | 6936 | GCFC2 | GC-rich sequence DNA-bindin... | XR_001738916.2 | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tcacaggccg aaaaggactt ttcggcagcg cgcggctgat tccagcgaca 121 gcgatggcgc cgaggagtcg cctgctgagc ctggggcgcc gagggaactt ccggtcccgg 181 gttctgcgga ggaagagccg ccctctggag gaggccgcgc gcaggtggcg ggactgcccc 241 accggattcg gggccctcgt ggccggggcc gggtctgggc gagctcccgg cgtgccacca 301 aagcggctcc ccgcgcggac gaaggctcag aatccagaac ccttgatgtg tccacagatg 361 aagaggataa aatacatcac tcctcagaaa gtaaggatga tcagggtttg tcttctgaca 421 gttctagctc tcttggagaa aaagaacttt catcaacagt taagatccca gatgcagctt 481 ttattcaggc agcccgcaga aaacgtgaat tggccagggc ccaagatgac tatatttctt 541 tggatgtaca acatacctcc tccatctctg gtatgaagag agagagcgaa gatgaccctg 601 agagtgagcc tgatgaccat gaaaagagaa taccatttac tctaagacct caaacactta 661 gacaaaggat ggctgaggaa tcaagtatgg atttaccaat atatgaagat tacccaactt 721 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 781 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta