Construct: ORF ccsbBroad304_11185

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014014.1_s304c1, BRDN0000411930
Derived from:
ccsbBroadEn_11185
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TEAD1 (7003)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11185
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7003 TEAD1 TEA domain transcription fa... NM_021961.6 82% 82% 1_45del;329_330insGGTAACAAGCAT;699_872del
2 mouse 21676 Tead1 TEA domain family member 1 NM_009346.3 78.7% 85.3% (many diffs)
3 mouse 21676 Tead1 TEA domain family member 1 NM_001166585.1 77.8% 84.3% (many diffs)
4 mouse 21676 Tead1 TEA domain family member 1 XM_006507563.2 77.8% 84.3% (many diffs)
5 mouse 21676 Tead1 TEA domain family member 1 XM_006507562.2 77.2% 83.3% (many diffs)
6 mouse 21676 Tead1 TEA domain family member 1 XM_011241723.1 76.4% 82.4% (many diffs)
7 mouse 21676 Tead1 TEA domain family member 1 NM_001166584.1 75% 81.1% (many diffs)
8 mouse 21676 Tead1 TEA domain family member 1 XM_006507561.2 74% 80.2% (many diffs)
9 mouse 21676 Tead1 TEA domain family member 1 XM_006507564.2 73.8% 80.2% (many diffs)
10 mouse 21676 Tead1 TEA domain family member 1 XM_006507565.2 72.8% 79.2% (many diffs)
11 mouse 21676 Tead1 TEA domain family member 1 XM_011241724.1 63.6% 66.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1140
ORF length:
1071
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaaaggatg agtgactctg cagataagcc aattgacaat gatgcagaag
121gggtctggag ccccgacatc gagcaaagct ttcaggaggc cctggctatc tatccaccat
181gtgggaggag gaaaatcatc ttatcagacg aaggcaaaat gtatggtagg aatgaattga
241tagccagata catcaaactc aggacaggca agacgaggac cagaaaacag gtgtctagtc
301acattcaggt tcttgccaga aggaaatctc gtgattttca ttccaagcta aaggtaacaa
361gcatggatca gactgcaaag gataaggccc tgcagcacat ggcggccatg tcctcagccc
421agatcgtctc ggccactgcc attcataaca agctggggct gcctgggatt ccacgcccga
481ccttcccagg ggcgccgggg ttctggccgg gaatgattca aacagggcag ccaggatcct
541cacaagacgt caagcctttt gtgcagcagg cctaccccat ccagccagcg gtcacagccc
601ccattccagg gtttgagcct gcatcggccc cagctccctc agtccctgcc tggcaaggtc
661gctccattgg cacaaccaag cttcgcctgg tggaattttc agcttttctc gagcagcagc
721gagacccaga ctcggctgat ttaaactgca atattcaaga tgatgctggg gctttttatg
781gtgtaaccag tcagtacgag agttctgaaa atatgacagt cacctgttcc accaaagttt
841gctcctttgg gaagcaagta gtagaaaaag tagagacgga gtatgcaagg tttgagaatg
901gccgatttgt ataccgaata aaccgctccc caatgtgtga atatatgatc aacttcatcc
961acaagctcaa acacttacca gagaaatata tgatgaacag tgttttggaa aacttcacaa
1021ttttattggt ggtaacaaac agggatacac aagaaactct actctgcatg gcctgtgtgt
1081ttgaagtttc aaatagtgaa cacggagcac aacatcatat ttacaggctt gtaaaggact
1141tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1201attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1261ggatta

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