Construct: ORF ccsbBroad304_11216

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017118.1_s304c1, BRDN0000410406
Derived from:
ccsbBroadEn_11216
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VAV1 (7409)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11216
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7409 VAV1 vav guanine nucleotide exch... NM_005428.4 93.1% 91.7% (many diffs)
2 human 7409 VAV1 vav guanine nucleotide exch... NM_001258206.2 90.5% 89.1% (many diffs)
3 human 7409 VAV1 vav guanine nucleotide exch... NM_001258207.2 89.3% 87.9% (many diffs)
4 human 7409 VAV1 vav guanine nucleotide exch... XM_005259642.1 87% 80% (many diffs)
5 mouse 22324 Vav1 vav 1 oncogene NM_001163815.1 82.2% 87.6% (many diffs)
6 mouse 22324 Vav1 vav 1 oncogene NM_011691.4 80% 85.1% (many diffs)
7 mouse 22324 Vav1 vav 1 oncogene NM_001163816.1 76.3% 81% (many diffs)
8 mouse 22324 Vav1 vav 1 oncogene XR_385313.2 48.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2436
ORF length:
2370
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat tgtgctacca ctgtactcca gactggacaa aagattcctg tgccttaaga
121acattagaac cttcctgtcc acctgctgtg agaagttcgg cctcaagcgg agcgagctct
181tcgaagcctt tgacctcttc gatgtgcagg attttggcaa ggtcatctac accctgtctg
241ctctgtcctg gaccccgatc gcccagaaca gggggatcat gcccttcccc accgaggagg
301agagtgtagg tgatgaagac atctacagtg gcctgtccga ccagatcgac gacacggtgg
361aggaggatga ggacctgtat gactgcgtgg agaatgagga ggcggaaggc gacgagatct
421atgaggacct catgcgctcg gagcccgtgt ccatgccgcc caagatgaca gagtatgaca
481agcgctgctg ctgcctgcgg gagatccagc agacggagga gaagtacact gacacgctgg
541gctccatcca gcagcatttc ttgaagcccc tgcaacggtt cctgaaacct caagacattg
601agatcatctt tatcaacatt gaggacctgc ttcgtgttca tactcacttc ctaaaggaga
661tgaaggaagc cctgggcacc cctggcgcag ccaatctcta ccaggtcttc atcaaataca
721aggagaggtt cctcgtctat ggccgctact gcagccaggt ggagtcagcc agcaaacacc
781tggaccgtgt ggccgcagcc cgggaggacg tgcagatgaa gctggaggaa tgttctcaga
841gagccaacaa cgggaggttc accctgcggg acctgctgat ggtgcctatg cagcgagttc
901tcaaatatca cctccttctc caggagctgg tgaaacacac gcaggaggcg atggagaagg
961agaacctgcg gctggccctg gatgccatga gggacctggc tcagtgcgtg aacgaggtca
1021agcgagacaa cgagacactg cgacagatca ccaatttcca gctgtccatt gagaacctgg
1081accagtctct ggctcactat ggccggccca agatcgacgg ggaactcaag atcacctcgg
1141tggaacggcg ctccaagatg gacaggtatg ccttcctgct cgacaaagct ctactcatct
1201gtaagcgcag gggagactcc tatgacctca aggactttgt aaacctgcac agcttccagg
1261ttcgggatga ctcttcagga gaccgagaca acaagaagtg gagccacatg ttcctcctga
1321tcgaggacca aggtgcccag ggctatgagc tgttcttcaa gacaagagaa ttgaagaaga
1381agtggatgga gcagtttgag atggccatct ccaacatcta tccggagaat gccaccgcca
1441acgggcatga cttccagatg ttctcctttg aggagaccac atcctgcaag gcctgtcaga
1501tgctgcttag aggtaccttc tatcagggct accgctgcca tcggtgccgg gcatctgcac
1561acaaggagtg tctggggagg gtccctccat gtggccgaca tgggcaagat ttcccaggaa
1621ctatgaagaa ggacaaacta catcgcaggg ctcaggacaa aaagaggaat gagctgggtc
1681tgcccaagat ggaggtgttt caggaatact acgggcttcc tccaccccct ggagccattg
1741gaccctttct acggctcaac cctggagaca ttgtggagct cacgaaggct gaggctgaac
1801agaactggtg ggagggcaga aatacatcta ctaatgaaat tggctggttt ccttgtaaca
1861gggtgaagcc ctatgtccat ggccctcctc aggacctgtc tgttcatctc tggtacgcag
1921gccccatgga gcgggcaggg gcagagagca tcctggccaa ccgctcggac gggactttct
1981tggtgcggca gagggtgaag gatgcagcag aatttgccat cagcattaaa tataacgtcg
2041aggtcaagca cattaaaatc atgacagcag aaggactgta ccggatcaca gagaaaaagg
2101ctttccgggg gcttacggag ctggtggagt tttaccagca gaactctcta aaggattgct
2161tcaagtctct ggacaccacc ttgcagttcc ccttcaagga gcctgaaaag agaaccatca
2221gcaggccagc agtgggaagc acaaagtatt ttggcacagc caaagcccgc tatgacttct
2281gcgcccgaga ccgatcagag ctgtcgctca aggagggtga catcatcaag atccttaaca
2341agaagggaca gcaaggctgg tggcgagggg agatctatgg ccgggttggc tggttccctg
2401ccaactacgt ggaggaagat tattctgaat actgctgccc aactttcttg tacaaagtgg
2461ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2521ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)