Transcript: Human XM_005259642.1

PREDICTED: Homo sapiens vav guanine nucleotide exchange factor 1 (VAV1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
VAV1 (7409)
Length:
2782
CDS:
101..2566

Additional Resources:

NCBI RefSeq record:
XM_005259642.1
NBCI Gene record:
VAV1 (7409)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005259642.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039860 CGTCGAGGTCAAGCACATTAA pLKO.1 2236 CDS 100% 13.200 18.480 N VAV1 n/a
2 TRCN0000286994 CGTCGAGGTCAAGCACATTAA pLKO_005 2236 CDS 100% 13.200 18.480 N VAV1 n/a
3 TRCN0000294447 GGCAGAAATACATCTACTAAT pLKO_005 2015 CDS 100% 13.200 10.560 N VAV1 n/a
4 TRCN0000010458 GACTGTACCGGATCACAGAGA pLKO.1 2274 CDS 100% 2.640 2.112 N VAV1 n/a
5 TRCN0000294401 GACTGTACCGGATCACAGAGA pLKO_005 2274 CDS 100% 2.640 2.112 N VAV1 n/a
6 TRCN0000294402 ACTACGTGGAGGAAGATTATT pLKO_005 2489 CDS 100% 15.000 10.500 N VAV1 n/a
7 TRCN0000039861 CCTCTTCGATGTGCAGGATTT pLKO.1 394 CDS 100% 10.800 7.560 N VAV1 n/a
8 TRCN0000039859 GCTCGACAAAGCTCTACTCAT pLKO.1 1378 CDS 100% 4.950 3.465 N VAV1 n/a
9 TRCN0000018362 TATGACTGCGTGGAGAATGAG pLKO.1 578 CDS 100% 4.950 3.465 N VAV1 n/a
10 TRCN0000039862 GTTTCCTTGTAACAGGGTGAA pLKO.1 2047 CDS 100% 4.050 2.835 N VAV1 n/a
11 TRCN0000039858 GCCTTGGCAGAGAGACGAGAA pLKO.1 2532 CDS 100% 1.350 0.945 N VAV1 n/a
12 TRCN0000286993 GCCTTGGCAGAGAGACGAGAA pLKO_005 2532 CDS 100% 1.350 0.945 N VAV1 n/a
13 TRCN0000339116 AGCCTTTGACCTCTTCGATTC pLKO_005 385 CDS 100% 6.000 4.200 N Cetn1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005259642.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11216 pDONR223 100% 87% 80% None (many diffs) n/a
2 ccsbBroad304_11216 pLX_304 0% 87% 80% V5 (many diffs) n/a
3 TRCN0000474410 ACAGGATACCATCCAACCTGATTT pLX_317 20.3% 87% 80% V5 (many diffs) n/a
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