Construct: ORF ccsbBroad304_11287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010579.1_s304c1, BRDN0000408412
- Derived from:
- ccsbBroadEn_11287
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPFF (8620)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11287
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8620 | NPFF | neuropeptide FF-amide pepti... | NM_001320296.2 | 100% | 100% | |
| 2 | human | 8620 | NPFF | neuropeptide FF-amide pepti... | NM_003717.4 | 75.3% | 65.3% | (many diffs) |
| 3 | human | 114108587 | ATF7-NPFF | ATF7-NPFF readthrough | NR_159377.1 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 417
- ORF length:
- 348
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttccgcag cctcctacca cttgcccctg gaagccagtc ccttcccctt 121 gtgacttacg tgtccagggt atttgcccat cttccttccc tgataccccc ttggcacagg 181 aggaagacag cgaacccctc ccaccacagg atgcccagac ctctgggtca ctgttgcact 241 acctgctcca ggcaatggag agacctggcc ggagccaagc cttcctgttt cagccccaga 301 ggtttggcag aaatacccag ggatcctgga ggaatgaatg gctgagtccc cgggctggag 361 aggggctgaa ttcccagttc tggagcctgg ctgcccctca acgctttggg aagaagttgc 421 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 481 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 541 tta