Construct: ORF ccsbBroad304_11364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012890.1_s304c1, BRDN0000408614
- Derived from:
- ccsbBroadEn_11364
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS6ST1 (9394)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11364
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9394 | HS6ST1 | heparan sulfate 6-O-sulfotr... | NM_004807.3 | 97.4% | 97.5% | 1_30del;177A>G |
| 2 | mouse | 50785 | Hs6st1 | heparan sulfate 6-O-sulfotr... | NM_015818.2 | 88.6% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1272
- ORF length:
- 1203
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttgagcgc gccagcaagt tcgtgctggt ggtggcgggc tcggtgtgct 121 tcatgctcat cttgtaccag tacgcgggcc caggactgag cctgggcgcg cccggcggcc 181 gcgcgccgcc cgacgacctg gacctgttcc ccacgcccga cccccactac gagaagaagt 241 actacttccc ggtccgcgag ctggagcgct cgctgcgctt cgacatgaag ggcgacgacg 301 tgatcgtctt cctgcacatc cagaagacgg gcggcaccac cttcggccgc cacctcgtgc 361 agaacgtacg cctcgaggtg ccgtgcgact gccggcccgg ccagaagaag tgcacctgct 421 accggcccaa ccgccgcgag acttggctct tctcccgctt ctccaccggc tggagctgcg 481 ggctgcacgc cgactggacc gagctcacca actgcgtgcc cggcgtgctg gaccgccgcg 541 actccgccgc gctgcgcacg cccaggaagt tctactacat caccctgcta cgagaccccg 601 tgtcccgcta cctgagcgag tggcggcatg tgcagagggg tgccacgtgg aagacgtcgt 661 tgcatatgtg tgatgggcgc acgcccacgc ctgaggagct gccgccctgc tacgagggca 721 cggactggtc gggctgcacg ctacaggagt tcatggactg cccgtacaac ctggccaaca 781 accgccaggt gcgcatgctg gccgacctga gcctggtggg ctgctacaac ctgtccttca 841 tccccgaggg caagcgggcc cagctgctgc tcgagagcgc caagaagaac ctgcggggca 901 tggccttctt cggcctgacc gagttccagc gcaagacgca gtacctgttc gagcggacgt 961 tcaacctcaa gttcatccgg cccttcatgc agtacaatag cacgcgggcg ggcggcgtgg 1021 aggtggatga agacaccatc cggcgcatcg aggagctcaa cgacctggac atgcagctgt 1081 acgactacgc caaggacctc ttccagcagc gctaccagta caagcggcag ctggagcgca 1141 gggagcagcg cctgaggagc cgcgaggagc gtctgctgca ccgggccaag gaggcactgc 1201 cgcgggagga tgccgacgag ccgggccgcg tgcccaccga ggactacatg agccacatca 1261 ttgagaagtg gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1321 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1381 caatcaacct ctggatta