Construct: ORF ccsbBroad304_11365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013330.1_s304c1, BRDN0000403299
- Derived from:
- ccsbBroadEn_11365
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SELENOF (9403)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11365
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9403 | SELENOF | selenoprotein F | NM_004261.5 | 38.1% | 0% | 1_256del;313_362del |
| 2 | human | 9403 | SELENOF | selenoprotein F | NM_203341.2 | 26% | 0% | 1_256del;372_373ins73 |
| 3 | human | 9403 | SELENOF | selenoprotein F | NR_144513.1 | 11.7% | 1_317del;374_423del;557_1603del | |
| 4 | human | 9403 | SELENOF | selenoprotein F | NR_144512.1 | 11.6% | 1_333del;390_439del;573_1619del | |
| 5 | mouse | 93684 | Selenof | selenoprotein F | NM_053102.2 | 35.3% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 255
- ORF length:
- 189
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggagctattc ttgaagtttg tggatgaaaa ttgggaaggt tccctcaagt 121 ccaagtatgt ccgtggttca gaccctgtat taaagctttt ggacgacaat gggaacattg 181 ctgaagaact gagcattctc aaatggaaca cagacagtgt agaagaattc ctgagtgaaa 241 agttggaacg catataccca actttcttgt acaaagtggt tggtaagcct atccctaacc 301 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 361 cgacaatcaa cctctggatt a