Construct: ORF ccsbBroad304_11426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002724.1_s304c1, BRDN0000407237
- Derived from:
- ccsbBroadEn_11426
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC24D (9871)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11426
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9871 | SEC24D | SEC24 homolog D, COPII coat... | NM_001318066.2 | 99.9% | 100% | 2397C>T |
| 2 | human | 9871 | SEC24D | SEC24 homolog D, COPII coat... | XM_005263379.3 | 99.9% | 100% | 2397C>T |
| 3 | human | 9871 | SEC24D | SEC24 homolog D, COPII coat... | NM_014822.4 | 99.8% | 99.9% | 672_673insGCA;2394C>T |
| 4 | human | 9871 | SEC24D | SEC24 homolog D, COPII coat... | XM_024454293.1 | 99.8% | 99.9% | 672_673insGCA;2394C>T |
| 5 | human | 9871 | SEC24D | SEC24 homolog D, COPII coat... | XM_017008875.1 | 56.8% | 56.9% | 0_1ins1335;1062C>T |
| 6 | mouse | 69608 | Sec24d | Sec24 related gene family, ... | NM_027135.2 | 83.5% | 88% | (many diffs) |
| 7 | mouse | 69608 | Sec24d | Sec24 related gene family, ... | XM_006502038.3 | 83.5% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3165
- ORF length:
- 3099
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tcaacaaggt tacgtggcta cacctccgta ttctcagcct cagcctggaa 121 taggcctttc tccacctcat tatgggcact atggggatcc gtcgcacaca gcatctccaa 181 caggtatgat gaagccagca gggcctttgg gggccaccgc cactagggga atgttgcctc 241 cgggtccccc acctcctgga ccccatcagt ttggtcagaa tggagctcat gccactggtc 301 accctcccca aagatttcca ggccctccac ctgtcaacaa tgtggcatcc tcacatgcac 361 cataccaacc ctctgcacaa tcttcttatc caggtcctat atccacttca tctgtcaccc 421 agctgggcag ccagctcagt gctatgcaaa tcaacagcta tggttcaggc atggctcctc 481 caagccaggg accccctggc cctctgtcag ccacatcatt gcagactcct ccacgacctc 541 cacagccttc cattttgcag cctggatctc aagttcttcc accaccaccc accacactca 601 atggtcctgg tgcctcacct ttgcctctac caatgtacag accagatggg ctctctgggc 661 ctcctcctcc aaatgcccag taccagcccc cacctcttcc aggccagacc ttgggtgctg 721 gatatcctcc gcagcaggca gccaactctg gtccccagat ggcaggcgca caactgtctt 781 acccaggagg cttccctgga ggtcctgcac agatggctgg tccgccacag ccccagaaga 841 agctggatcc tgactctatc cctagcccaa tccaggtgat tgagaatgat agagccagca 901 gaggaggaca agtttatgcc accaacacca gaggccagat ccctcccctg gtcactacag 961 attgcatgat acaagaccaa ggaaatgcca gtcctcgatt catccgttgt acaacatact 1021 gttttccatg cacgtcagat atggctaagc aagctcagat tccattagct gctgtcatca 1081 agccctttgc caccattcct tcaaatgaga gtccccttta cttggtaaat cacggcgaga 1141 gtggaccagt cagatgcaac aggtgcaagg cctacatgtg cccatttatg cagttcatcg 1201 aaggaggaag gagatatcag tgtggatttt gcaactgtgt gaatgatgtt ccaccattct 1261 atttccaaca tctggaccac attggaagaa gactggacca ctatgagaaa ccagagttat 1321 ctctaggatc ttatgaatat gttgccactt tggattattg cagaaagagt aagcctccca 1381 acccaccagc ctttatcttc atgattgatg tttcatatag taacataaag aatggacttg 1441 tcaagctcat atgtgaagaa ctgaagacca tgctggaaaa aattccaaag gaagagcaag 1501 aagagacgtc tgcaattcga gtgggtttta tcacatataa caaagttctc catttcttta 1561 atgtgaagag taatctggcc cagcctcaga tgatggtggt gactgatgtt ggagaagtct 1621 ttgttccttt gttggatggt ttccttgtca actatcaaga atcccaatct gtgattcata 1681 atttgttgga ccagattcca gacatgtttg cagactctaa tgaaaatgag actgtctttg 1741 ctcctgtcat ccaggctggc atggaagcac taaaggcagc agactgtcct gggaagctgt 1801 tcatcttcca ttcttccttg ccaactgctg aagcaccagg gaagctcaaa aacagagatg 1861 acaaaaaact ggttaataca gacaaagaga agatactttt ccagccccaa acaaatgtct 1921 atgactcatt ggccaaggac tgcgtggctc acggctgctc tgtgacactc ttcctctttc 1981 ctagtcagta tgtggacgtg gcctcgctgg ggctggttcc tcagctcact ggaggaaccc 2041 tttacaaata caacaatttc cagatgcact tggatagaca acaatttttg aacgacctca 2101 gaaatgatat tgaaaagaaa ataggctttg atgctattat gagggttcgt accagcacag 2161 gtttcagagc cactgatttc tttggtggaa tcttgatgaa caacaccacc gatgtagaaa 2221 tggctgccat cgattgtgac aaggcagtga ccgtggagtt caagcacgat gacaaactca 2281 gtgaagacag tggagcctta atccagtgtg ctgtgcttta cacgacaatc agtggtcaaa 2341 gaagacttcg gattcacaat cttggcttaa actgcagctc tcagctagct gatctttata 2401 agagctgtga gacagatgct cttatcaact tctttgccaa gtcagctttt aaagcagttc 2461 ttcaccagcc tttgaaggtc atccgggaaa ttctagttaa tcagactgcc catatgttgg 2521 catgttaccg gaagaattgt gcaagtcctt ctgcagcaag ccagcttatt ctaccagatt 2581 ccatgaaagt attgccagtg tacatgaatt gcttgttgaa aaactgtgta ctactcagca 2641 gaccagagat ctcaactgat gaacgagcat accagagaca gctggtcatg accatgggtg 2701 tggctgactc tcagcttttc ttctacccac aacttctgcc catacacacg ttagatgtca 2761 agagtacaat gttacctgct gccgttcgtt gctctgagtc ccgtctttca gaagaaggaa 2821 tattcttact ggctaatggt ctacacatgt tcctgtggtt gggagtaagc agcccaccag 2881 aactgatcca aggaatattt aatgtgccat cttttgcaca tatcaacaca gatatgacat 2941 tgctgcctga agtgggaaac ccatactctc aacaactcag aatgataatg ggtattatcc 3001 aacaaaagag gccatattca atgaagctca caattgtaaa gcagcgagaa caaccagaaa 3061 tggttttccg acagttcctg gtagaagaca aaggacttta cggaggctct tcttatgtgg 3121 atttcctttg ttgtgttcac aaggagatct gtcagctgct taattaccca actttcttgt 3181 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 3241 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a