Construct: ORF ccsbBroad304_11451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005199.1_s304c1, BRDN0000406633
- Derived from:
- ccsbBroadEn_11451
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1M2 (10053)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11451
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10053 | AP1M2 | adaptor related protein com... | NM_001300887.2 | 99.8% | 99.7% | 615A>G;1096A>C |
| 2 | human | 10053 | AP1M2 | adaptor related protein com... | NM_005498.5 | 99.3% | 99% | 615A>G;673_674insTTTCAG;1090A>C |
| 3 | human | 10053 | AP1M2 | adaptor related protein com... | XM_024451304.1 | 84% | 83.7% | 200_439del;855A>G;1336A>C |
| 4 | human | 10053 | AP1M2 | adaptor related protein com... | XM_024451303.1 | 83.6% | 83.1% | (many diffs) |
| 5 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | NM_001110300.1 | 87.1% | 96.9% | (many diffs) |
| 6 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | NM_009678.2 | 86.6% | 96.2% | (many diffs) |
| 7 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | XM_006509949.3 | 79.7% | 88.7% | (many diffs) |
| 8 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | XM_006509950.3 | 79.3% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcctcggct gtcttcattc tggacgttaa gggcaagcca ttgatcagcc 121 gcaactacaa gggcgatgtg gccatgagca agattgagca cttcatgcct ttgctggtac 181 agcgggagga ggaaggcgcc ctggccccgc tgctgagcca cggccaggtc cacttcctat 241 ggatcaaaca cagcaacctc tacttggtgg ccaccacatc gaagaatgcc aatgcctccc 301 tggtgtactc cttcctgtat aagacaatag aggtattctg cgaatacttc aaggagctgg 361 aggaggagag catccgggac aactttgtca tcgtctacga gttgctggac gagctcatgg 421 actttggctt cccgcagacc accgacagca agatcctgca ggagtacatc actcagcaga 481 gcaacaagct ggagacgggc aagtcacggg tgccacccac tgtcaccaac gctgtgtcct 541 ggcgctccga gggtatcaag tataagaaga acgaggtctt cattgatgtc atagagtctg 601 tcaacctgct ggtcaatgcc aacggcagcg tccttctgag cgaaatcgtc ggtaccatca 661 agctcaaggt gtttctgtcg ggaatgccag agctgcggct gggcctcaat gaccgcgtgc 721 tcttcgagct cactggcctt tcaggcagca agaacaaatc agtagagctg gaggatgtaa 781 aattccacca gtgcgtgcgg ctctctcgct ttgacaacga ccgcaccatc tccttcatcc 841 cgcctgatgg tgactttgag ctcatgtcat accgcctcag cacccaggtc aagccactga 901 tctggattga gtctgtcatt gagaagttct cccacagccg cgtggagatc atggtcaagg 961 ccaaggggca gtttaagaaa cagtcagtgg ccaacggtgt ggagatatct gtgcctgtac 1021 ccagcgatgc cgactccccc agattcaaga ccagtgtggg cagcgccaag tatgtgccgg 1081 agagaaacgt cgtgatttgg agtattaagt ctttcccggg gggcaaggag tacttgatgc 1141 gagcccactt tggcctcccc cgtgtggaaa aggaagaggt ggagggccgg ccccccatcg 1201 gggtcaagtt tgagatcccc tacttcaccg tctctgggat ccaggtccga tacatgaaga 1261 tcattgagaa aagtggttac caggccctgc cctgggttcg ctacatcacc cagagtggcg 1321 attaccaact tcgtaccagc tacccaactt tcttgtacaa agtggttggt aagcctatcc 1381 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1441 ttaagtcgac aatcaacctc tggatta