Construct: ORF ccsbBroad304_11470

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007217.1_s304c1, BRDN0000411058
Derived from:
ccsbBroadEn_11470
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX39A (10212)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11470
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10212 DDX39A DExD-box helicase 39A NM_005804.4 75.4% 68% 864_973del;1077_1281del
2 human 10212 DDX39A DExD-box helicase 39A XM_011527620.1 75.4% 68% 864_973del;1077_1281del
3 human 10212 DDX39A DExD-box helicase 39A NR_046366.2 66.7% 1_118del;1085_1448del
4 human 10212 DDX39A DExD-box helicase 39A XM_024451310.1 34.8% 23.7% (many diffs)
5 human 10212 DDX39A DExD-box helicase 39A XM_006722606.4 27.4% 21.2% 0_1ins615;249_358del;462_666del
6 human 10212 DDX39A DExD-box helicase 39A XM_011527621.3 27.4% 21.2% 0_1ins615;249_358del;462_666del
7 human 10212 DDX39A DExD-box helicase 39A XM_024451311.1 27.4% 21.2% 0_1ins615;249_358del;462_666del
8 human 10212 DDX39A DExD-box helicase 39A XM_024451312.1 27.4% 21.2% 0_1ins615;249_358del;462_666del
9 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... NM_197982.3 67.4% 65.9% (many diffs)
10 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_006531332.3 67.4% 65.9% (many diffs)
11 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312949.1 62.8% 61.6% (many diffs)
12 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312950.1 62.8% 61.6% (many diffs)
13 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312952.1 25.6% 21.2% (many diffs)
14 mouse 68278 Ddx39 DEAD (Asp-Glu-Ala-Asp) box ... XM_017312951.1 23.9% 19.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1032
ORF length:
966
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agaacaggat gtggaaaacg atcttttgga ttacgatgaa gaggaagagc
121cccaggctcc tcaagagagc acaccagctc cccctaagaa agacatcaag ggatcctacg
181tttccatcca cagctctggc ttccgggact ttctgctgaa gccggagctc ctgcgggcca
241tcgtggactg tggctttgag catccttctg aggtccagca tgagtgcatt ccccaggcca
301tcctgggcat ggacgtcctg tgccaggcca agtccgggat gggcaagaca gcggtcttcg
361tgctggccac cctacagcag attgagcctg tcaacggaca ggtgacggtc ctggtcatgt
421gccacacgag ggagctggcc ttccagatca gcaaggaata tgagcgcttt tccaagtaca
481tgcccagcgt caaggtgtct gtgttcttcg gtggtctctc catcaagaag gatgaagaag
541tgttgaagaa gaactgtccc catgtcgtgg tggggacccc gggccgcatc ctggcgctcg
601tgcggaatag gagcttcagc ctaaagaatg tgaagcactt tgtgctggac gagtgtgaca
661agatgctgga gcagctggac atgcggcggg atgtgcagga gatcttccgc ctgacaccac
721acgagaagca gtgcatgatg ttcagcgcca ccctgagcaa ggacatccgg cctgtgtgca
781ggaagttcat gcaggatccc atggaggtgt ttgtggacga cgagaccaag ctcacgctgc
841acggcctgca gcagtactac gtcaaactca aagacagtga gaagaaccgc aagctctttg
901atctcttgga tgtgctggag tttaaccagc ctgtcacgct atcagcagtt caaggatttc
961cagcggcgga tcctggtggc caccaatctg tttggccggg ggatggacat cgagcgagtc
1021aacatcgtct ttacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1081tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1141caatcaacct ctggatta

Download FASTA (ORF) (Full)