Construct: ORF ccsbBroad304_11500

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015455.1_s304c1, BRDN0000410091
Derived from:
ccsbBroadEn_11500
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ECI2 (10455)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11500
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_001166010.1 98.6% 98.6% 1_15del
2 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_006117.2 98.6% 98.6% 1_15del
3 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_206836.2 91.1% 91.1% 1_105del
4 human 10455 ECI2 enoyl-CoA delta isomerase 2 XM_017010170.1 90.3% 90.3% 1_15del;704_705ins90
5 human 10455 ECI2 enoyl-CoA delta isomerase 2 XM_006714957.2 83.5% 83.5% 1_105del;794_795ins90
6 human 10455 ECI2 enoyl-CoA delta isomerase 2 NR_028588.1 72.7% 1_132del;527_597del;1281_1481del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1143
ORF length:
1077
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag agccagtcag aaggactttg aaaattcaat gaatcaagtg aaactcttga
121aaaaggatcc aggaaacgaa gtgaagctaa aactctacgc gctatataag caggccactg
181aaggaccttg taacatgccc aaaccaggtg tatttgactt gatcaacaag gccaaatggg
241acgcatggaa tgcccttggc agcctgccca aggaagctgc caggcagaac tatgtggatt
301tggtgtccag tttgagtcct tcattggaat cctctagtca ggtggagcct ggaacagaca
361ggaaatcaac tgggtttgaa actctggtgg tgacctccga agatggcatc acaaagatca
421tgttcaaccg gcccaaaaag aaaaatgcca taaacactga gatgtatcat gaaattatgc
481gtgcacttaa agctgccagc aaggatgact caatcatcac tgttttaaca ggaaatggtg
541actattacag tagtgggaat gatctgacta acttcactga tattccccct ggtggagtag
601aggagaaagc taaaaataat gccgttttac tgagggaatt tgtgggctgt tttatagatt
661ttcctaagcc tctgattgca gtggtcaatg gtccagctgt gggcatctcc gtcaccctcc
721ttgggctatt cgatgccgtg tatgcatctg acagggcaac atttcataca ccatttagtc
781acctaggcca aagtccggaa ggatgctcct cttacacttt tccgaagata atgagcccag
841ccaaggcaac agagatgctt atttttggaa agaagttaac agcgggagag gcatgtgctc
901aaggacttgt tactgaagtt ttccctgata gcacttttca gaaagaagtc tggaccaggc
961tgaaggcatt tgcaaagctt cccccaaatg ccttgagaat ttcaaaagag gtaatcagga
1021aaagagagag agaaaaacta cacgctgtta atgctgaaga atgcaatgtc cttcagggaa
1081gatggctatc agatgaatgc acaaatgctg tggtgaactt cttatccaga aaatcaaaac
1141tgtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1201tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1261tctggatta

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