Transcript: Human XM_017010170.1

PREDICTED: Homo sapiens enoyl-CoA delta isomerase 2 (ECI2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ECI2 (10455)
Length:
1297
CDS:
112..1116

Additional Resources:

NCBI RefSeq record:
XM_017010170.1
NBCI Gene record:
ECI2 (10455)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017010170.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049387 GATCTGACTAACTTCACTGAT pLKO.1 622 CDS 100% 4.950 3.960 N ECI2 n/a
2 TRCN0000289680 GATCTGACTAACTTCACTGAT pLKO_005 622 CDS 100% 4.950 3.960 N ECI2 n/a
3 TRCN0000049384 GCCATAAACACTGAGATGTAT pLKO.1 508 CDS 100% 5.625 3.938 N ECI2 n/a
4 TRCN0000289681 GCCATAAACACTGAGATGTAT pLKO_005 508 CDS 100% 5.625 3.938 N ECI2 n/a
5 TRCN0000049386 GCTATCAGATGAATGCACAAA pLKO.1 1056 CDS 100% 4.950 3.465 N ECI2 n/a
6 TRCN0000307109 GCTATCAGATGAATGCACAAA pLKO_005 1056 CDS 100% 4.950 3.465 N ECI2 n/a
7 TRCN0000049385 CCAGGAAACGAAGTGAAGCTA pLKO.1 190 CDS 100% 3.000 2.100 N ECI2 n/a
8 TRCN0000306850 CCAGGAAACGAAGTGAAGCTA pLKO_005 190 CDS 100% 3.000 2.100 N ECI2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017010170.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02442 pDONR223 100% 91.7% 91.7% None 704_705ins90 n/a
2 ccsbBroad304_02442 pLX_304 0% 91.7% 91.7% V5 704_705ins90 n/a
3 TRCN0000470211 TCTGTACGATAGGTTCAACATTTG pLX_317 40.1% 91.7% 91.7% V5 704_705ins90 n/a
4 ccsbBroadEn_11500 pDONR223 100% 90.3% 90.3% None 1_15del;704_705ins90 n/a
5 ccsbBroad304_11500 pLX_304 0% 90.3% 90.3% V5 1_15del;704_705ins90 n/a
6 TRCN0000479728 CATTACGGAAGAAAATTAACAGTG pLX_317 33.7% 90.3% 90.3% V5 1_15del;704_705ins90 n/a
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