Construct: ORF ccsbBroad304_11505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011852.1_s304c1, BRDN0000461717
- Derived from:
- ccsbBroadEn_11505
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIBF1 (10464)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11505
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10464 | PIBF1 | progesterone immunomodulato... | NM_006346.4 | 91.2% | 90.3% | (many diffs) |
| 2 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_024449314.1 | 90.8% | 87.6% | (many diffs) |
| 3 | human | 10464 | PIBF1 | progesterone immunomodulato... | NM_001349655.2 | 87.9% | 87% | (many diffs) |
| 4 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_011534882.3 | 87.2% | 84.2% | (many diffs) |
| 5 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_011534884.3 | 83.9% | 83.7% | (many diffs) |
| 6 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_001749459.2 | 81.6% | (many diffs) | |
| 7 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_001749457.2 | 76.6% | (many diffs) | |
| 8 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_017020351.2 | 75.8% | 75.5% | (many diffs) |
| 9 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_017020350.2 | 75% | 74.1% | (many diffs) |
| 10 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_001749458.2 | 74.7% | (many diffs) | |
| 11 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_001749456.2 | 74.2% | (many diffs) | |
| 12 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_002957449.1 | 73.6% | (many diffs) | |
| 13 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_011534885.3 | 72.2% | 71.3% | (many diffs) |
| 14 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_001749460.2 | 69% | (many diffs) | |
| 15 | human | 10464 | PIBF1 | progesterone immunomodulato... | NR_146206.2 | 68.1% | (many diffs) | |
| 16 | human | 10464 | PIBF1 | progesterone immunomodulato... | XR_941461.3 | 66.8% | (many diffs) | |
| 17 | human | 10464 | PIBF1 | progesterone immunomodulato... | NR_146205.2 | 66.8% | (many diffs) | |
| 18 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_011534886.3 | 60.7% | 60.5% | (many diffs) |
| 19 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_017020352.2 | 40.3% | 36.1% | (many diffs) |
| 20 | human | 10464 | PIBF1 | progesterone immunomodulato... | XM_017020354.2 | 38.4% | 37.5% | (many diffs) |
| 21 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | NM_029320.3 | 79.8% | 82% | (many diffs) |
| 22 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XM_006519251.3 | 71.2% | 72.4% | (many diffs) |
| 23 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XR_383193.3 | 67.5% | (many diffs) | |
| 24 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XM_006519253.3 | 66% | 67.8% | (many diffs) |
| 25 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XM_006519254.3 | 65.6% | 67.3% | (many diffs) |
| 26 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XM_006519255.3 | 65.1% | 62.8% | (many diffs) |
| 27 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XR_001781130.1 | 62.3% | (many diffs) | |
| 28 | mouse | 52023 | Pibf1 | progesterone immunomodulato... | XM_006519256.3 | 55.7% | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2163
- ORF length:
- 2094
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctcgaaaa atttcaaagg agtcaaaaaa agtgaacatc tctagttctc 121 tggaatctga agatattagt ttagaaacaa cagttcctac ggatgatatt tcctcatcag 181 aagagcgaga gggcaaagtc agaatcacca ggcagctaat tgaacgaaaa gaactacttc 241 ataatattca gttactaaaa attgagctat cccagaaaac tatgatgatc gacaatttga 301 aagtggatta tcttacaaag attgaagaat tggaggagaa acttaatgat gcacttcacc 361 agaagcagct actaacattg agattagaca accaattggc ttttcaacag aaagatgcca 421 gcaaatatca agaattaatg aaacaagaaa tggaaaccat tttgttgaga cagaaacaac 481 tagaagagac aaatcttcag ctaagagaaa aagctggaga tgttcgtcga aacctgcgtg 541 actttgagtt gacagaagag caatatgtta aattaaaagc ttttcctgaa gatcagcttt 601 ctattcctga atatgtatct gttcgcttct atgagctagt gaatccatta agaaaggaaa 661 tctgtgaact acaagtgaaa aagaatatcc tagcagaaga attaagtaca aacaaaaacc 721 aactgaagca gctgacagag acatatgagg aagatcgaaa aaactactct gaagttcaaa 781 ttagatgtca acgtttggcc ttagaattag cagacacaaa acagttaatt cagcaaggtg 841 actaccgtca agagaactat gataaagtca agagtgaacg tgatgcactt gaacaggaag 901 taattgagct taggagaaaa catgaaatac ttgaagcctc tcacatgatt caaacaaaag 961 aacgaagtga attatcaaaa gaggtagtca ccttagagca aactgttact ttactgcaaa 1021 aggataaaga atatcttaat cgccaaaaca tggagcttag tgttcgctgt gctcatgaag 1081 aggatcgcct tgaaagactt caagctcaac tggaagaaag caaaaaggct agagaagaga 1141 tgtatgaaaa atatgtagca tccagagacc attataaaac agaatatgaa aataaactac 1201 atgatgaact agaacaaatc agattgaaaa ccaaccaaga aattgatcaa cttcgaaatg 1261 cctctaggga aatgtatgaa cgagaaaaca gaaatctccg agaagcaagg gataatgctg 1321 tggctgaaaa ggaacgagca gtgatggctg aaaaggatgc tttagaaaaa cacgatcagc 1381 tcttagacag gtacagagaa ctacaactta gtacagaaag caaagtaaca gaatttctcc 1441 atcaaagtaa attaaaatct tttgaaagtg agcgtgttca acttctgcaa gaggaaacag 1501 caagaaatct cacacagtgt caattggaat gtgaaaaata tcaggaaaaa ttggaggttt 1561 taaccaaaga attttatagt ctccaagcct cttctgaaaa acgcattact gaacttcaag 1621 cacagaactc agagcatcaa gcaaggctag acatttatga gaaactggaa aaagagcttg 1681 atgaaataat aatgcaaact gcagaaattg aaaatgaaga tgaggctgaa agggttcttt 1741 tttcctacgg ctatggtgct aatgttccca caacagccaa aagacgacta aagcaaagtg 1801 ttcacttggc aagaagagtg cttcaattag aaaaacaaaa ctcgctgatt ttaaaagatc 1861 tggaacatcg aaaggaccaa gtaacacagc tttcacaaga gcttgacaga gccaattcgc 1921 tattaaacca gactcaacag ccttacaggt atctcgttga atcagtgcgt cagagagatt 1981 ctaagattga ttcactgacg gaatctattg cacaacttga gaaagatgtc agcaacttaa 2041 ataaagaaaa gtcagcttta ctacagacga agaatcaaat ggcattagat ttagaacaac 2101 ttctaaatca tcgtgaggta tttttcctat tttgtcataa ttgtaatcat tttaggcttt 2161 ttttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2221 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2281 tctggatta