Construct: ORF ccsbBroad304_11518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014618.1_s304c1, BRDN0000409553
- Derived from:
- ccsbBroadEn_11518
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P3H3 (10536)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11518
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10536 | P3H3 | prolyl 3-hydroxylase 3 | NM_014262.5 | 74.8% | 74.8% | 1_555del |
| 2 | mouse | 14789 | P3h3 | prolyl 3-hydroxylase 3 | NM_013534.4 | 64.1% | 65.3% | (many diffs) |
| 3 | mouse | 14789 | P3h3 | prolyl 3-hydroxylase 3 | XM_017321394.1 | 63% | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1719
- ORF length:
- 1653
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca cctgcagatg cgggaggaca tggctaagta cagacgaatg tcgggagttc 121 ggccccagag cttccgggac ctggagacgc ccccacactg ggcagcctat gacactggcc 181 tggagctact ggggcgccag gaggcaggac tggcactgcc caggctagag gaggctcttc 241 aggggagcct ggcccagatg gagagctgcc gtgctgactg tgaggggcct gaggagcagc 301 agggggctga agaagaggag gatggggctg cgagccaggg gggcctctat gaggccattg 361 caggacactg gattcaggtc ctgcagtgcc ggcaacgctg tgtgggggaa acagccacac 421 gccctggtcg cagcttccct gtcccagact tccttcccaa ccagctgagg cggctacatg 481 aggcccatgc tcaggtgggc aatctgtccc aggctataga aaatgtcctg agtgtcctgc 541 tcttctaccc ggaggatgag gctgccaaga gggctctgaa ccagtaccag gcccagctgg 601 gagagccgag acctggcctc ggacccagag aggacatcca gcgcttcatc ctccgatccc 661 tgggggagaa gaggcagctc tactatgcca tggagcacct ggggaccagc ttcaaggatc 721 ctgacccctg gacccctgca gctctcatcc ctgaggcact tagagaaaag ctcagagagg 781 atcaagagaa gaggccttgg gaccatgagc ccgtgaagcc aaagcccttg acctactgga 841 aggatgtcct tctcctggag ggtgtgacct tgacccagga ttccaggcag ctgaatgggt 901 cggagcgggc ggtgttggat gggctgctca ccccagccga gtgtggggtg ctgctgcagc 961 tggctaagga tgcagctggg gctggagcca ggtctggcta tcgtggtcgc cgctcccctc 1021 acacccccca tgaacgcttc gaggggctca cggtgcttaa ggctgcgcag ctggcccggg 1081 ctgggacagt gggcagtcag ggtgctaagc tgcttctgga ggtgagcgag cgggtgcgga 1141 ccttgaccca ggcctacttc tccccggaac ggcccctgca tctgtccttc acccacctgg 1201 tgtgccgcag cgccatagaa ggagagcaag agcagcgcat ggacctgagt cacccagtgc 1261 acgcagacaa ctgcgtcctg gaccctgaca cgggagagtg ctggcgggag cccccagcct 1321 acacctatcg ggactacagc ggactcctct acctcaacga tgacttccag ggtggggacc 1381 tgttcttcac ggagcccaac gccctcactg tcacggctcg ggtgcgtcct cgctgtgggc 1441 gccttgtggc cttcagctcc ggtgtcgaga atccccatgg ggtgtgggcc gtgactcggg 1501 gacggcgctg tgccctggca ctgtggcaca cgtgggcacc tgagcacagg gagcaggagt 1561 ggatagaagc caaagaactg ctgcaggagt cacaggagga ggaggaagag gaagaggaag 1621 aaatgcccag caaagaccct tccccagagc cccctagccg caggcaccag agggtccaag 1681 acaagactgg aagggcacct cgggttcggg aggagctgtg cccaactttc ttgtacaaag 1741 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1801 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta