Construct: ORF ccsbBroad304_11586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005731.1_s304c1, BRDN0000405672
- Derived from:
- ccsbBroadEn_11586
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPSF6 (11052)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11586
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | NM_001300947.2 | 100% | 100% | |
| 2 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | XM_005268588.3 | 99.8% | 99.8% | 1578_1580delAAG |
| 3 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | NM_007007.3 | 93.7% | 93.7% | 692_693ins111 |
| 4 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | XM_005268590.3 | 93.5% | 93.5% | 692_693ins111;1467_1469delAAG |
| 5 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513854.3 | 93% | 99.6% | (many diffs) |
| 6 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513855.2 | 93% | 99.6% | (many diffs) |
| 7 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513851.3 | 92.9% | 99.4% | (many diffs) |
| 8 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513852.2 | 92.9% | 99.4% | (many diffs) |
| 9 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513853.2 | 92.9% | 99.4% | (many diffs) |
| 10 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | NM_001013391.2 | 86.7% | 93.3% | (many diffs) |
| 11 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513858.3 | 86.7% | 93.3% | (many diffs) |
| 12 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | NM_001310609.1 | 86.6% | 93.2% | (many diffs) |
| 13 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513856.3 | 86.6% | 93.2% | (many diffs) |
| 14 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XR_001779551.1 | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1830
- ORF length:
- 1764
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacggcgtg gaccacatag acatttacgc ggatgtcggc gaagagttca 121 accaggaagc tgaatatggt gggcatgatc agatagattt gtatgacgat gtcatatctc 181 catctgcaaa taatggagat gccccagaag accgagatta catggatact ctcccaccaa 241 ctgttggtga tgatgtgggt aaaggagcag caccaaatgt tgtctataca tatactggaa 301 agagaattgc attatatatt ggaaatctaa catggtggac aacagatgaa gacttaactg 361 aagcagttca ttctttggga gtaaatgata ttttggagat aaaatttttt gaaaatcggg 421 caaatggcca gtcaaagggg tttgcccttg ttggtgttgg atctgaagca tcttcaaaaa 481 agttaatgga tctgttacct aaaagagaac ttcatggtca gaatcctgtt gtaactccat 541 gcaataaaca gttcctgagt caatttgaaa tgcagtccag gaaaactaca caatcaggac 601 aaatgtctgg ggaaggtaaa gctggtcctc caggaggcag ttcccgtgca gcatttccac 661 aaggtggtag aggacggggc cgttttccag gggctgttcc tggtggggac agatttcctg 721 ggccagcagg accaggaggg ccacccccac cttttccagg aaatttgatc aagcatcttg 781 ttaaaggaac tcggcctttg ttcctggaaa ctaggattcc atggcatatg gggcacagca 841 tagaggaaat acccattttt ggcctaaaag ctggacagac tccaccacgt ccacccttag 901 gtcctccagg cccacctggt ccaccaggtc ctccacctcc tggtcaggtt ctgcctcctc 961 ctctagctgg gcctcctaat cgaggagatc gccctccacc accagttctt tttcctggac 1021 aaccttttgg gcagcctcca ttgggtccac ttcctcctgg ccctccacct ccagttccag 1081 gctacggccc ccctcctggc ccaccacctc cacaacaggg accacctcca cctccaggcc 1141 cctttccacc tcgtccaccc ggtccacttg ggccacccct tacactagct cctcctccgc 1201 atcttcctgg accacctcca ggtgccccac cgccagctcc gcatgtgaac ccagctttct 1261 ttcctccacc aactaacagt ggcatgccta catcagatag ccgaggtcca ccaccaacag 1321 atccatatgg gcgacctcca ccatatgata ggggtgacta tggcccccct ggaagggaaa 1381 tggatactgc aagaacgcca ttgagtgaag ctgaatttga agaaatcatg aatagaaata 1441 gggcaatctc aagcagtgct atttcgagag ctgtgtctga tgccagtgct ggtgattatg 1501 ggagtgctat tgagacactg gtaactgcaa tttctttaat taaacaatcc aaagtatctg 1561 ctgatgatcg ttgcaaagtt cttattagtt ctttgcaaga ttgccttcat ggaattgagt 1621 ccaagtctta tggttctgga tcaagacgtg aacgatcaag agagagggac catagtagat 1681 cacgagaaaa gagtcgacgt cataaatccc gtagtagaga ccgtcatgac gattattaca 1741 gagagagaag cagagaacga gagaggcacc gggatcgtga ccgagaccgt gaccgagagc 1801 gtgaccgaga gcgcgaatat cgtcatcgtt acccaacttt cttgtacaaa gtggttggta 1861 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1921 ggtggcgcgt taagtcgaca atcaacctct ggatta