Construct: ORF ccsbBroad304_11588

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005178.1_s304c1, BRDN0000408157
Derived from:
ccsbBroadEn_11588
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DUS4L (11062)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11588
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11062 DUS4L dihydrouridine synthase 4 like NM_001270419.1 61.8% 61.8% 1_363del
2 human 11062 DUS4L dihydrouridine synthase 4 like NM_181581.3 61.8% 61.8% 1_363del
3 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NM_001371365.2 33% 28.1% (many diffs)
4 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NM_001371366.2 33% 28.1% (many diffs)
5 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NM_001371367.2 33% 28.1% (many diffs)
6 human 11062 DUS4L dihydrouridine synthase 4 like NR_073005.1 29.2% 1_458del;1047_2012del
7 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NM_001371364.2 27.2% 23.1% (many diffs)
8 human 11062 DUS4L dihydrouridine synthase 4 like NR_073003.1 27.1% 1_608del;1197_2162del
9 human 11062 DUS4L dihydrouridine synthase 4 like NR_073004.1 25.2% 1_608del;721_722ins43;1154_2119del
10 human 11062 DUS4L dihydrouridine synthase 4 like NR_073002.1 24.7% 1_826del;1415_2380del
11 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NR_163941.2 7.3% (many diffs)
12 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NR_163940.2 7.2% (many diffs)
13 human 115253422 DUS4L-BCAP29 DUS4L-BCAP29 readthrough NR_163939.2 7.2% (many diffs)
14 mouse 71916 Dus4l dihydrouridine synthase 4-l... XM_006515224.2 84.4% 85.2% (many diffs)
15 mouse 71916 Dus4l dihydrouridine synthase 4-l... XM_006515225.3 84.4% 85.2% (many diffs)
16 mouse 71916 Dus4l dihydrouridine synthase 4-l... XM_006515223.2 70.2% 70.9% (many diffs)
17 mouse 71916 Dus4l dihydrouridine synthase 4-l... NM_028002.2 52.8% 53.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
657
ORF length:
588
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagaaggt tatggggctt gcttaataaa caagccagag cttgttcaag
121acatggtgaa acaagtaaga aatcaagtgg aaacccctgg attttcagtt tctattaaaa
181taaggatcca tgatgacctt aaaagaactg tagatctttg tcaaaaggct gaagcaacag
241gagtttcatg gattacagtc catggaagaa ctgctgaaga aagacatcag ccagtgcact
301atgattccat taaaataatt aaggaaaata tgtctatacc tgtaattgct aatggagaca
361tcagaagctt aaaggaagca gaaaatgtgt ggcggattac tgggacagat ggtgtgatgg
421ttgcaagagg actcttagca aacccggcca tgtttgctgg atatgaggaa accccactga
481aatgcatctg ggactgggtt gacattgctc ttgaactcgg gactccttac atgtgtttcc
541atcaacattt aatgtacatg atggaaaaga taacttcaag gcaggaaaaa agggtattta
601atgctctgtc aagcacatca gcaatcatag attaccttac agaccattat ggcattttgc
661caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
721ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
781tta

Download FASTA (ORF) (Full)