Construct: ORF ccsbBroad304_11588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005178.1_s304c1, BRDN0000408157
- Derived from:
- ccsbBroadEn_11588
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUS4L (11062)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11588
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NM_001270419.1 | 61.8% | 61.8% | 1_363del |
| 2 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NM_181581.3 | 61.8% | 61.8% | 1_363del |
| 3 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NM_001371365.2 | 33% | 28.1% | (many diffs) |
| 4 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NM_001371366.2 | 33% | 28.1% | (many diffs) |
| 5 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NM_001371367.2 | 33% | 28.1% | (many diffs) |
| 6 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NR_073005.1 | 29.2% | 1_458del;1047_2012del | |
| 7 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NM_001371364.2 | 27.2% | 23.1% | (many diffs) |
| 8 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NR_073003.1 | 27.1% | 1_608del;1197_2162del | |
| 9 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NR_073004.1 | 25.2% | 1_608del;721_722ins43;1154_2119del | |
| 10 | human | 11062 | DUS4L | dihydrouridine synthase 4 like | NR_073002.1 | 24.7% | 1_826del;1415_2380del | |
| 11 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NR_163941.2 | 7.3% | (many diffs) | |
| 12 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NR_163940.2 | 7.2% | (many diffs) | |
| 13 | human | 115253422 | DUS4L-BCAP29 | DUS4L-BCAP29 readthrough | NR_163939.2 | 7.2% | (many diffs) | |
| 14 | mouse | 71916 | Dus4l | dihydrouridine synthase 4-l... | XM_006515224.2 | 84.4% | 85.2% | (many diffs) |
| 15 | mouse | 71916 | Dus4l | dihydrouridine synthase 4-l... | XM_006515225.3 | 84.4% | 85.2% | (many diffs) |
| 16 | mouse | 71916 | Dus4l | dihydrouridine synthase 4-l... | XM_006515223.2 | 70.2% | 70.9% | (many diffs) |
| 17 | mouse | 71916 | Dus4l | dihydrouridine synthase 4-l... | NM_028002.2 | 52.8% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 657
- ORF length:
- 588
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaaggt tatggggctt gcttaataaa caagccagag cttgttcaag 121 acatggtgaa acaagtaaga aatcaagtgg aaacccctgg attttcagtt tctattaaaa 181 taaggatcca tgatgacctt aaaagaactg tagatctttg tcaaaaggct gaagcaacag 241 gagtttcatg gattacagtc catggaagaa ctgctgaaga aagacatcag ccagtgcact 301 atgattccat taaaataatt aaggaaaata tgtctatacc tgtaattgct aatggagaca 361 tcagaagctt aaaggaagca gaaaatgtgt ggcggattac tgggacagat ggtgtgatgg 421 ttgcaagagg actcttagca aacccggcca tgtttgctgg atatgaggaa accccactga 481 aatgcatctg ggactgggtt gacattgctc ttgaactcgg gactccttac atgtgtttcc 541 atcaacattt aatgtacatg atggaaaaga taacttcaag gcaggaaaaa agggtattta 601 atgctctgtc aagcacatca gcaatcatag attaccttac agaccattat ggcattttgc 661 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 721 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 781 tta