Construct: ORF ccsbBroad304_11625

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010016.1_s304c1, BRDN0000399226
Derived from:
ccsbBroadEn_11625
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NID2 (22795)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11625
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22795 NID2 nidogen 2 NM_007361.4 70.3% 70.1% (many diffs)
2 human 22795 NID2 nidogen 2 XM_005267405.5 68.9% 68.8% (many diffs)
3 human 22795 NID2 nidogen 2 XM_005267407.5 66.8% 66.6% (many diffs)
4 human 22795 NID2 nidogen 2 XM_005267406.5 65.5% 65.3% (many diffs)
5 mouse 18074 Nid2 nidogen 2 NM_008695.2 58.4% 59% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2973
ORF length:
2907
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gggggaccgg gtggccgggc ggccggtgct gtcgtcgtta ccagtgctac
121tgctgctgca gttgctaatg ttgcgggccg cggcgctgca cccagacgag ctcttcccac
181acggggagtc gtggggggac cagctcctgc aggaaggcga cgacgaaagc tcagccgtgg
241tgaagctggc gaatcccctg cacttctacg aagcccgatt cagcaacctc tacgtgggca
301ccaacggcat catctccact caggacttcc ccagggaaac gcagtatgtg gactatgatt
361tccccaccga cttcccggcc atcgcccctt ttctggcgga catcgacacg agccacggca
421gaggccgagt cctgtaccga gtgaatggga aagtgagtgg ccacctccac gtgggccata
481cacccgtgca cttcactgat gtggacctgc atgcgtatat cgtgggcaat gatggcagag
541cctacacggc catcagccac atcccacagc cagcagccca ggccctcctc cccctcacac
601caattggagg cctgtttggc tggctctttg ctttagaaaa acctggctct gagaacggct
661tcagcctcgc aggtgctgcc tttacccatg acatggaagt tacattctac ccgggagagg
721agacggttcg tatcactcaa actgctgagg gacttgaccc agagaactac ctgagcatta
781agaccaacat tcaaggccag gtgccttacg tcccagcaaa tttcacagcc cacatctctc
841cctacaagga gctgtaccac tactccgact ccactgtgac ctctacaagt tccagagact
901actctctgac ttttggtgca atcaaccaaa catggtccta ccgcatccac cagaacatca
961cttaccaggt gtgcaggcat gcccccagac acccgtcctt ccccaccacc cagcagctga
1021acgtggaccg ggtctttgcc ttgtataatg acgaagaaag agtgcttaga tttgctgtga
1081ccaatcaaat tggcccggtc aaagaagatt cagaccccac tccggtgaat ccttgctatg
1141atgggagcca catgtgtgac acaacagcac ggtgccatcc agggacaggt gtagattaca
1201cctgtgagtg cgcatctggg taccagggag atggacggaa ctgtgtggat gaaaatgaat
1261gtgcaactgg ctttcatcgc tgtggcccca actctgtatg tatcaacttg cctggaagct
1321acaggtgtga gtgccggagt gcttatgagt ttgcagatga ccggcatact tgcatcttga
1381tcaccccacc tgccaacccc tgtgaggatg gcagtcatac ctgtgctcct gctgggcagg
1441cccggtgtgt tcaccatgga ggcagcacgt tcagctgtgc ctgcctgcct ggttatgccg
1501gcgatgggca ccagtgcact gatgtagatg aatgctcaga aaacagatgt caccctgcag
1561ctacctgcta caatactcct ggttccttct cctgccgttg tcaacccgga tattatgggg
1621atggatttca gtgcatacct gactccacct caagcctgac accctgtgaa caacagcagc
1681gccatgccca ggcccagtat gcctaccctg gggcccggtt ccacatcccc caatgcgacg
1741agcagggcaa cttcctgccc ctacagtgtc atggcagcac tggtttctgc tggtgcgtgg
1801accctgatgg tcatgaagtt cctggtaccc agactccacc tggctccacc ccgcctcact
1861gtggaccatc accagagccc acccagaggc ccccgaccat ctgtgagcgc tggagggaaa
1921acctgctgga gcactacggt ggcacccccc gggatgacca gtacgtgccc cagtgcgatg
1981acctgggcca cttcatcccc ctgcagtgcc acggaaagag cgacttctgc tggtgtgtgg
2041acaaagatgg cagagaggtg cagggcaccc gctcccagcc aggcaccacc cctgcgtgta
2101tacccaccgt cgctccaccc atggtccggc ccacgccccg gccagatgtg acccctccat
2161ctgtgggcac cttcctgctc tatactcagg gccagcagat tggctactta cccctcaatg
2221gcaccaggct tcagaaggat gcagctaaga ccctgctgtc tctgcatggc tccataatcg
2281tgggaattga ttacgactgc cgggagagga tggtgtactg gacagatgtt gctggacgga
2341caatcagccg tgccggtctg gaactgggag cagagcctga gacgatcgtg aattcaggtc
2401tgataagccc tgaaggactt gccatagacc acatccgcag aacaatgtac tggacggaca
2461gtgtcctgga taagatagag agcgccctgc tggatggctc tgagcgcaag gtcctcttct
2521acacagatct ggtgaatccc cgtgccatcg ctgtggatcc aatccgaggc aacttgtact
2581ggacagactg gaatagagaa gctcctaaaa ttgaaacgtc atctttagat ggagaaaaca
2641gaagaattct gatcaataca gacattggat tgcccaatgg cttaaccttt gaccctttct
2701ctaaactgct ctgctgggca gatgcaggaa ccaaaaaact ggagtgtaca ctacctgatg
2761gaactggacg gcgtgtcatt caaaacaacc tcaagtaccc cttcagcatc gtaagctatg
2821cagatcactt ctaccacaca gactggagga gggatggtgt tgtatcagta aataaacata
2881gtggccagtt tactgatgag tatctcccag aacaacgatc tcacctctac gggataactg
2941cagtctaccc ctactgccca acaggaagaa agtacccaac tttcttgtac aaagtggttg
3001gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
3061accggtggcg cgttaagtcg acaatcaacc tctggatta

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