Transcript: Human XM_005267405.5

PREDICTED: Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NID2 (22795)
Length:
4951
CDS:
66..4274

Additional Resources:

NCBI RefSeq record:
XM_005267405.5
NBCI Gene record:
NID2 (22795)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005267405.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000054228 CCGGCATACTTGCATCTTGAT pLKO.1 2660 CDS 100% 4.950 6.930 N NID2 n/a
2 TRCN0000054231 CCGGGTCTTTGCCTTGTATAA pLKO.1 2327 CDS 100% 13.200 9.240 N NID2 n/a
3 TRCN0000291351 CCGGGTCTTTGCCTTGTATAA pLKO_005 2327 CDS 100% 13.200 9.240 N NID2 n/a
4 TRCN0000054232 CGCAGTATGTGGACTATGATT pLKO.1 340 CDS 100% 5.625 3.938 N NID2 n/a
5 TRCN0000291350 CGCAGTATGTGGACTATGATT pLKO_005 340 CDS 100% 5.625 3.938 N NID2 n/a
6 TRCN0000054229 CCCTTCAGCATCGTAAGCTAT pLKO.1 4098 CDS 100% 4.950 3.465 N NID2 n/a
7 TRCN0000333616 CCCTTCAGCATCGTAAGCTAT pLKO_005 4098 CDS 100% 4.950 3.465 N NID2 n/a
8 TRCN0000114781 CCTCAATCTTTACTACTGTAT pLKO.1 4441 3UTR 100% 4.950 3.465 N Nid2 n/a
9 TRCN0000336463 CCTCAATCTTTACTACTGTAT pLKO_005 4441 3UTR 100% 4.950 3.465 N Nid2 n/a
10 TRCN0000054230 GCTGATGATCTGAAGTCAGAA pLKO.1 759 CDS 100% 4.950 3.465 N NID2 n/a
11 TRCN0000291352 GCTGATGATCTGAAGTCAGAA pLKO_005 759 CDS 100% 4.950 3.465 N NID2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005267405.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11625 pDONR223 100% 68.9% 68.8% None (many diffs) n/a
2 ccsbBroad304_11625 pLX_304 0% 68.9% 68.8% V5 (many diffs) n/a
3 TRCN0000492085 ATCACAATGGGTACAGTACTCTGA pLX_317 15.2% 68.9% 68.8% V5 (many diffs) n/a
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