Construct: ORF ccsbBroad304_11643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014887.1_s304c1, BRDN0000409881
- Derived from:
- ccsbBroadEn_11643
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KHDC4 (22889)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11643
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22889 | KHDC4 | KH domain containing 4, pre... | NM_014949.4 | 38.1% | 37% | (many diffs) |
| 2 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | NM_028814.3 | 34.9% | 35.2% | (many diffs) |
| 3 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XM_017319752.1 | 34% | 34.3% | (many diffs) |
| 4 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XR_375592.1 | 22.2% | (many diffs) | |
| 5 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XR_375591.1 | 22% | (many diffs) | |
| 6 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XR_375589.1 | 21.4% | (many diffs) | |
| 7 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XR_867238.1 | 17% | (many diffs) | |
| 8 | mouse | 74200 | 2810403A07Rik | RIKEN cDNA 2810403A07 gene | XR_001783760.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcggggagc gcgacacatc ctggagctgg cgggcgccgc agcaaatggg 121 accaaccagc tccagcccca cttctcttcc tcccgccagc ggccccaggt ggggaggtca 181 ccagcagtgg gggaagtcct gggggcacca cagctgctcc ttcaggagcc ttggatgctg 241 ctgctgctgt ggctgccaag attaatgcca tgctcatggc aaaagggaag ctgaaaccaa 301 ctcagaatgc ttctgagaag cttcaggctc ctggcaaagg cctaactagc aataaaagca 361 aggatgacct ggtggtagct gaagtagaaa ttaatgatgt gcctctcaca tgtaggaact 421 tgctgactcg aggacagact caagacgaga tcagccgact tagtggggct gcagtatcaa 481 ctcgagggag gttcatgaca actgaggaaa aagccaaagt gggaccaggg gatcgtccat 541 tatatcttca tgttcagggc cagacacggg aattagtgga cagagctgta aaccggatca 601 aagaaattat caccaatgga gtggtaaaag ctgccacagg aacaagtcca acttttaatg 661 gtgcaacagt aactgtctat caccagccag cacccatcgc tcagttgtct ccagctgtta 721 gccagaagcc tcccttccag tcaggggtat gttttgggga gggatcgtta atagcattat 781 catttctata cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 841 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 901 tcaacctctg gatta