Construct: ORF ccsbBroad304_11664

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002596.1_s304c1, BRDN0000462417
Derived from:
ccsbBroadEn_11664
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF21B (23046)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11664
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23046 KIF21B kinesin family member 21B NM_001252102.2 100% 100%
2 human 23046 KIF21B kinesin family member 21B NM_001252103.2 99.1% 99.1% 3803_3804ins39
3 human 23046 KIF21B kinesin family member 21B NM_001252100.2 98.8% 98.2% (many diffs)
4 human 23046 KIF21B kinesin family member 21B NM_017596.4 98% 97.4% (many diffs)
5 human 23046 KIF21B kinesin family member 21B XM_017000731.1 95.4% 94.8% (many diffs)
6 human 23046 KIF21B kinesin family member 21B XM_017000732.1 74.3% 73.6% (many diffs)
7 mouse 16565 Kif21b kinesin family member 21B NM_001039472.1 88.7% 96% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4938
ORF length:
4869
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccggccag ggggactgct gcgtcaaggt ggccgtcagg atccggcccc
121agctgtcgaa ggagaagatt gagggctgtc acatctgtac ctctgttacc ccgggagagc
181cccaggtcct gctggggaag gacaaggcct tcacctatga ctttgtcttc gacctggaca
241cctggcaaga acagatctat tccacctgtg tgagcaagct catcgagggc tgcttcgagg
301gctataatgc cacggtgctg gcctatgggc agacgggggc cgggaagacg tacaccatgg
361gcactggctt tgacatggca acgtcggagg aggagcaggg catcatcccg agggccatcg
421cacacctctt tgggggcatt gccgagcgca agcgccgggc acaggagcag ggcgtggctg
481gacctgagtt caaagtcagc gcccagtttc tggagctcta caacgaggag atccttgacc
541tgtttgacag cacccgtgac cctgacaccc gccaccgcag gtccaacatc aagatccacg
601aggacgcaaa cggtggcatc tacaccactg gcgtcacttc tcgcctcatc cactcccagg
661aggagctgat ccagtgcctg aagcaggggg ccctgtcccg caccacagcc agcacccaga
721tgaacgtgca gagctcacgc tcccacgcca tcttcaccat ccacctgtgc cagatgcgca
781tgtgcaccca gcccgacctg gtgaatgagg cggtgactgg gcttcctgat ggtacacctc
841cctcgagtga gtatgagaca ctcactgcta agtttcactt tgtggacctg gccggctcag
901agcggctgaa gcggacaggg gctactggcg agcgggccaa ggagggcatc tccatcaact
961gtggcctgct ggccttgggc aatgtgatca gcgccttagg ggaccagagc aagaaggtgg
1021tgcacgttcc ctacagggac tccaagctca ctcggctcct ccaggattcg ctggggggca
1081acagccagac catcatgatc gcctgtgtga gcccctcaga ccgagatttc atggagaccc
1141tcaacacact caaatatgcc aatcgggccc gcaacatcaa gaacaaggtg gtagtgaacc
1201aggacaagac cagccagcaa atcagtgcac tgcgggctga gattgctcgg ctgcagatgg
1261agctgatgga gtataaggcg ggcaagcgag tgataggaga ggatggcgct gagggctata
1321gtgatctgtt ccgagagaat gccatgctac agaaggagaa tggggccctg cggctgcggg
1381tgaaagccat gcaggaggcc atcgatgcca tcaacaaccg cgtcacccag ctcatgagcc
1441aggaggccaa cctgctgcta gccaaggccg gcgatggcaa tgaggccatt ggtgcgctga
1501tccagaacta catccgggag atcgaggagc tacggactaa gcttctagag agtgaagcca
1561tgaacgagtc cctgcgccgc agcctctcac gggcctcggc taggagcccc tactccctgg
1621gtgcttctcc agccgccccg gccttcgggg gcagccctgc cagctccatg gaggatgcct
1681cggaggtgat ccgcagggcc aagcaggacc tggagcggct aaagaagaag gaggtcaggc
1741agcggaggaa gagccccgag aaggaagcct tcaaaaagag ggcaaaactc caacaggaga
1801acagcgagga gacggatgag aacgaggcgg aggaggagga ggaagagcga gacgagagtg
1861gctgtgagga ggaggaaggg cgcgaggatg aagatgagga ctcgggcagt gaagagagcc
1921tggtggactc agactcagac cccgaggaga aggaggtgaa cttccaggcg gacctggccg
1981acctgacttg tgagatcgaa atcaagcaga agctgatcga cgagctggag aacagccagc
2041ggcggttgca gacgctcaag caccagtatg aggaaaagct gattctgctg cagaacaaga
2101tccgagacac acagctggag cgcgaccgtg tgctgcagaa cctcagcacc atggagtgct
2161atactgagga gaaggccaac aagatcaagg cagactatga gaagaggctg cgggagatga
2221accgggacct gcagaagctg caggccgccc agaaagagca cgcccggctg cttaagaacc
2281agtcgcgcta cgagagggag ctgaagaagc tacaggccga ggtggctgag atgaagaagg
2341ccaaggtggc cctgatgaag cagatgcgtg aggagcaaca gcggcggcgg ctagtggaga
2401ccaagaggaa ccgggagatc gcacagctca agaaggagca gcggcgacag gagtttcaga
2461tccgagctct ggagtcccag aagcggcagc aggagatggt cctgaggagg aagacccagg
2521aggtttctgc actgaggcgc ctggccaagc ccatgtctga gcgggtggca gggcgtgcag
2581gactaaagcc acccatgctg gactctgggg ctgaggtgtc ggccagcact acctcatctg
2641aggctgaatc aggggcccgc tctgtctcca gcatcgtgcg ccagtggaac cgcaaaatca
2701accacttctt gggggaccat cctgcgccca ctgtcaatgg cacccgtcct gcccgaaaga
2761agttccagaa gaagggggcc agccagagct tcagtaaggc ggcaaggctc aagtggcagt
2821ccctggagcg acggatcatt gacatcgtca tgcagagaat gaccattgtc aacctggagg
2881ctgacatgga gcggctcatc aagaaaaggg aggagctgtt cctcctgcag gaggcactgc
2941ggaggaagcg ggagcggctg caggctgaga gccccgagga agagaagggg ctgcaggagc
3001tggctgagga gatcgaggtg ctggcagcca acattgacta catcaatgac ggcatcaccg
3061actgccaggc caccatcgtg cagctggagg agaccaagga ggagctggac tccacagaca
3121catccgtggt catcagctcc tgctccctgg ctgaagcccg cctcctgcta gacaacttcc
3181tcaaggcatc cattgacaag gggctgcaag tggcacaaaa ggaagcccag atccggctgt
3241tggagggccg actgaggcag acggatatgg caggctcctc ccagaaccat ctgctcctgg
3301acgccctgcg tgagaaggct gaagctcacc ccgagctgca ggccctcatc tacaatgtgc
3361agcaggagaa tggctacgcc agcacagatg aggagatctc agagttctct gagggcagct
3421tctcccagtc attcaccatg aaaggctcca ccagccatga cgatttcaag ttcaagagcg
3481agcccaaact gtctgcccaa atgaaagctg tgtcggctga gtgcctgggc cccccactgg
3541atatctccac caagaacatc accaagtccc tggcctccct cgttgagatc aaagaggacg
3601gagtgggctt ctctgtccga gacccctatt accgggacag ggtctcgcgc accgtcagtc
3661tgcctacccg gggcagcact ttccctaggc aatctcgagc cacagagacg tccccgctga
3721cgagaaggaa gtcctacgac cgagggcagc ccattaggtc cacagatgtg ggattcacac
3781ccccatcatc ccctcccact cggccccgca atgaccgcaa tgtcttctct cgtctcacca
3841gtaatcagag ccaggggtca gcgctggaca agtctgatga cagcgactcc tctttgtcgg
3901aggtcctgag gggcatcatc tccccggttg gaggagccaa gggtgcacgg acggccccac
3961tgcagtgtgt ctccatggcc gaaggccaca ccaagcccat cctctgcctg gatgccacag
4021atgagttgct attcacaggg tccaaagacc gaagctgcaa gatgtggaac ttggttacgg
4081gacaggagat cgcagctcta aagggccacc ccaacaacgt ggtctccatc aagtactgca
4141gccactcggg gcttgtgttc tccgtgtcca cctcctacat caaggtgtgg gacatccggg
4201actcagccaa gtgcattcgg actctcacgt cctcgggcca ggtgatctca ggggatgcct
4261gtgccgccac atccacccgt gccatcacca gtgctcaggg cgagcatcag atcaaccaga
4321tcgccctcag cccttcgggc accatgctgt acgccgcctc gggcaatgcc gtccgcatct
4381gggagcttag caggttccag cctgtcggca agctgactgg ccacatcggc cctgtgatgt
4441gcctgacggt cacccagacg gccagccagc atgacctcgt ggtgactggc tccaaggacc
4501actacgttaa gatgttcgag ctgggcgagt gtgtgacggg caccatcggc cccactcaca
4561acttcgagcc cccgcactac gatggcatcg agtgtctcgc catccaggga gacatcctgt
4621tcagtggctc ccgagataac ggcatcaaga agtgggacct agaccagcag gagctcatcc
4681agcaaatccc caatgcgcac aaggactggg tgtgcgccct ggccttcatc ccgggccgcc
4741ccatgctgct cagcgcctgc cgtgcgggtg tcatcaaggt ctggaacgtg gacaacttca
4801cacccatcgg tgagatcaag ggccacgaca gtcccatcaa tgccatctgc accaatgcca
4861agcatatctt cacagcctcc agtgacctga cggtgaagtt ctggagtgtc cggcggttac
4921cccacagcgg cccacccttg ccaactttct tgtacaaagt ggttggtaag cctatcccta
4981accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
5041agtcgacaat caacctctgg atta

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