Construct: ORF ccsbBroad304_11668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003634.1_s304c1, BRDN0000402375
- Derived from:
- ccsbBroadEn_11668
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D9B (23061)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11668
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23061 | TBC1D9B | TBC1 domain family member 9B | NM_015043.4 | 31.6% | 31.5% | 1_2526del;2743G>T;3305A>C |
| 2 | human | 23061 | TBC1D9B | TBC1 domain family member 9B | NM_198868.3 | 31.2% | 31.1% | (many diffs) |
| 3 | mouse | 76795 | Tbc1d9b | TBC1 domain family, member 9B | NM_029745.2 | 26.9% | 28.1% | (many diffs) |
| 4 | mouse | 76795 | Tbc1d9b | TBC1 domain family, member 9B | NM_001290759.1 | 26.7% | 27.8% | (many diffs) |
| 5 | mouse | 76795 | Tbc1d9b | TBC1 domain family, member 9B | NM_001290760.1 | 26.6% | 27.7% | (many diffs) |
| 6 | mouse | 76795 | Tbc1d9b | TBC1 domain family, member 9B | XM_006534458.3 | 25.9% | 27.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggnatggc cggccgtcgg gaccccagcc tgccctacct ggagcagtac cggattgatg 121 ccagccagtt ccgggaactc tttgccagcc tgacaccctg ggcctgtggc tcccacacac 181 ctctgctggc agggcgcatg ttcaggctcc tggacgaaaa caaggactcg ctgatcaact 241 tcaaggagtt cgtgacaggg atgagcggga tgtaccacgg gtacctgaca gagaagctca 301 aggtgctcta caagctacac cttcccccag ctctgagccc agaggaagcc gagtcagccc 361 tggaggcggc ccattatttc acagaggaca gctcctcaga agaagcacta ccacaggaag 421 agcaagaagg aagtggaagt gaggagagag gagaggagaa ggggaccagc tctccggact 481 atcggcacta ccttcgaatg tgggccaagg agaaagaggc tcagaaggag acgattaagg 541 atcttcccaa gatgaaccag gagcagttca ttgagctgtg caagacgctt tacaacatgt 601 tcagtgaaga ccccatggag caggacctgt accacgccat cgccaccgtg gccagcctcc 661 tgctccgcat cggagaggtg gggaagaagt tctcagcccg cacaggcagg aagcccaggg 721 actgtgccac tgaggaggac gagccaccag cacccgaact gcatcaggac gcagccaggg 781 agcttcagcc cccagctgca ggagaccccc aagccaaagc aggcggagac acacacctcg 841 gaacagcccc acaggagagc caggtggtgg tggagggggg cagcggcgag ggacagggct 901 caccctccca gctgctgtct gacgatgaaa ccaaagacga catgtccatg tcctcctact 961 cggtggtcag cacgggctcc ctgcaatgtg aagaccttgc agacgacacg gtgctggtgg 1021 gcggggaggc ctgcagcccc acagcgcgca tcggcggcac cgtcgacacc gactggtgca 1081 tctcctttga gcagatcctg gcctccatcc tgacggagtc cgtgctggtg aacttctttg 1141 agaagagagt ggacattgga ctcaagatca aggaccaaaa gaaagtggag agacagttca 1201 gcaccgccag tgaccatgag cagcctggag tttccggctg atcaactttc ttgtacaaag 1261 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1321 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta