Construct: ORF ccsbBroad304_11692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009764.1_s304c1, BRDN0000462233
- Derived from:
- ccsbBroadEn_11692
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GANAB (23193)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11692
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001278194.1 | 99.9% | 99.7% | 907C>T;1090C>T |
| 2 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001329222.1 | 99.9% | 99.7% | 907C>T;1090C>T |
| 3 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001329223.2 | 99.9% | 99.7% | 907C>T;1090C>T |
| 4 | human | 23193 | GANAB | glucosidase II alpha subunit | XM_017017412.1 | 99.9% | 99.7% | 907C>T;1090C>T |
| 5 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001278193.2 | 97.4% | 96.1% | (many diffs) |
| 6 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001278192.2 | 95% | 93.6% | (many diffs) |
| 7 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_198334.3 | 89.6% | 89.5% | 1_291del;1198C>T;1381C>T |
| 8 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_198335.4 | 87.6% | 87.4% | (many diffs) |
| 9 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001329224.1 | 82.9% | 82.7% | 0_1ins432;475C>T;658C>T |
| 10 | human | 23193 | GANAB | glucosidase II alpha subunit | NM_001329225.2 | 82.9% | 82.7% | 0_1ins432;475C>T;658C>T |
| 11 | mouse | 14376 | Ganab | alpha glucosidase 2 alpha n... | XM_006526678.2 | 87.5% | 91.6% | (many diffs) |
| 12 | mouse | 14376 | Ganab | alpha glucosidase 2 alpha n... | XM_006526677.2 | 85.3% | 89.2% | (many diffs) |
| 13 | mouse | 14376 | Ganab | alpha glucosidase 2 alpha n... | NM_001293621.1 | 78.5% | 82.2% | (many diffs) |
| 14 | mouse | 14376 | Ganab | alpha glucosidase 2 alpha n... | NM_008060.2 | 76.7% | 80.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2610
- ORF length:
- 2541
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcggttc aggattgatg agctggagcc tcggcgaccc cgataccgtg 121 taccagatgt tttggtggct gatccaccaa tagcccggct ttctgtctct ggtcgtgatg 181 agaacagtgt ggagttaacc atggctgagg gaccctacaa gatcatcttg acagcacggc 241 cattccgcct tgacctacta gaggaccgaa gtcttttgct tagtgtcaat gcccgaggac 301 tcttggagtt tgagcatcag agggccccta gggtctcgca aggatcaaaa gacccagctg 361 agggcgatgg ggcccagcct gaggaaacac ccagggatgg cgacaagcca gaggagactc 421 aggggaaggc agagaaagat gagccaggag cctgggagga gacattcaaa actcactctg 481 acagcaagcc gtatggcccc atgtctgtgg gtttggactt ctctctgcca ggcatggagc 541 atgtctatgg gatccctgag catgcagaca acctgaggct gaaggtcact gagggtgggg 601 agccatatcg cctctacaat ttggatgtgt tccagtatga gctgtacaac ccaatggcct 661 tgtatgggtc tgtgcctgtg ctcctggcac acaaccctca tcgcgacttg ggcatcttct 721 ggctcaatgc tgcagagacc tgggttgata tatcttccaa cactgccggg aagaccctgt 781 ttgggaagat gatggactac ctgcagggct ctggggagac cccacagaca gatgttcgct 841 ggatgtcaga gactggcatc attgacgtct tcctgctgct ggggccctcc atctctgatg 901 ttttccggca atatgctagt ctcacaggaa cccaggcgtt gcccccactc ttctccctcg 961 gctaccacca gagctgttgg aactaccggg acgaggctga tgtgctggaa gtggatcagg 1021 gctttgatga tcacaacctg ccctgtgatg tcatctggct agacattgaa catgctgatg 1081 gcaagcggta tttcacctgg gaccccagtc gcttccctca gccccgcacc atgcttgagc 1141 gcttggcttc taagaggtgg aagctggtgg ccatcgtaga cccccacatc aaggtggact 1201 ccggctaccg agttcacgag gagctgcgga acctggggct gtatgttaaa acccgggatg 1261 gctctgacta tgagggctgg tgctggccag gctcagctgg ttaccctgac ttcactaatc 1321 ccacgatgag ggcctggtgg gctaacatgt tcagctatga caattatgag ggctcagctc 1381 ccaacctctt tgtctggaat gacatgaacg aaccatctgt gttcaatggt cctgaggtca 1441 ccatgctcaa ggatgcccag cattatgggg gctgggagca ccgggatgtg cataacatct 1501 atggccttta tgtgcacatg gcgactgctg atgggctgag acagcgctct gggggcatgg 1561 aacgcccctt tgtcctggcc agggccttct tcgctggctc ccagcgcttt ggagccgtgt 1621 ggacagggga caacactgcc gagtgggacc atttgaagat ctctattcct atgtgtctca 1681 gcttggggct ggtgggactt tccttctgtg gggcggatgt gggtggcttc ttcaaaaacc 1741 cagagccaga gctgcttgtg cgctggtacc agatgggtgc ttaccagcca ttcttccggg 1801 cacatgccca cttggacact gggcgacgag agccatggct gttaccatct cagcacaatg 1861 atataatccg agatgccttg ggccagcgat attctttgct gcccttctgg tacaccctct 1921 tatatcaggc ccatcgggaa ggcattcctg tcatgaggcc cctgtgggtg cagtaccctc 1981 aggatgtgac taccttcaat atagatgatc agtacttgct tggggatgcg ttgctggttc 2041 accctgtatc agactctgga gcccatggtg tccaggtcta tctgcctggc caaggggagg 2101 tgtggtatga cattcaaagc taccagaagc atcatggtcc ccagaccctg tacctgcctg 2161 taactctaag cagtatccct gtgttccagc gtggagggac aatcgtgcct cgatggatgc 2221 gagtgcggcg gtcttcagaa tgtatgaagg atgaccccat cactctcttt gttgcactta 2281 gccctcaggg tacagctcaa ggagagctct ttctggatga tgggcacacg ttcaactatc 2341 agactcgcca agagttcctg ctgcgtcgat tctcattctc tggcaacacc cttgtctcca 2401 gctcagcaga ccctgaagga cactttgaga caccaatctg gattgagcgg gtggtgataa 2461 taggggctgg aaagccagca gctgtggtac tccagacaaa aggatctcca gaaagccgcc 2521 tgtccttcca gcatgaccct gagacctctg tgttggtcct gcgcaagcct ggcatcaatg 2581 tggcatctga ttggagtatt cacctgcgat tgccaacttt cttgtacaaa gtggttggta 2641 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2701 ggtggcgcgt taagtcgaca atcaacctct ggatta