Construct: ORF ccsbBroad304_11785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005171.1_s304c1, BRDN0000408017
- Derived from:
- ccsbBroadEn_11785
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR5A (25938)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11785
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25938 | HEATR5A | HEAT repeat containing 5A | NM_015473.4 | 5.5% | 5.5% | 339_6138delinsG |
| 2 | mouse | 320487 | Heatr5a | HEAT repeat containing 5A | NM_177171.4 | 4.8% | 5% | (many diffs) |
| 3 | mouse | 320487 | Heatr5a | HEAT repeat containing 5A | XM_006516012.3 | 4.8% | 5% | (many diffs) |
| 4 | mouse | 320487 | Heatr5a | HEAT repeat containing 5A | XM_006516011.3 | 4.7% | 4.9% | (many diffs) |
| 5 | mouse | 320487 | Heatr5a | HEAT repeat containing 5A | XM_006516009.3 | 4.7% | 4.9% | (many diffs) |
| 6 | mouse | 320487 | Heatr5a | HEAT repeat containing 5A | XM_006516010.2 | 4.7% | 4.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga attagctcat agcttactgc tgaatgaaga agcatacaat caactaggtg 121 aagttcagaa ggcagagttt atttttgagt ggttgagata cttggagaag ctcttgttgg 181 caaccagcag gaatgatgta agggagaaac agaagactct tgttgaacag ctcctgtctt 241 tgttgaacag ctccccaggg cctcctaccc gcaaactgct tgctaagaat ctagccatac 301 tttatagtat tggagacaca ttctccgttc atgaagcaat cgataaatgt aatgatctta 361 ttcgtagcaa agatgattct ccaagttatc ttcccactaa gctgtaccca actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a