Construct: ORF ccsbBroad304_11802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005000.1_s304c1, BRDN0000462085
- Derived from:
- ccsbBroadEn_11802
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHG3 (26030)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11802
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021158.2 | 64% | 64% | 1_1263del;2623C>A |
| 2 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | NM_001308147.2 | 61.6% | 61.5% | 1_1401del;1403A>T;2761C>A |
| 3 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021157.2 | 61.6% | 61.5% | 1_1401del;1403A>T;2761C>A |
| 4 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_024449528.1 | 61.6% | 61.5% | 1_1401del;1403A>T;2761C>A |
| 5 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536629.3 | 60.1% | 60% | 1_1491del;1493A>T;2851C>A |
| 6 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021154.2 | 56% | 56% | 1_1764del;3124C>A |
| 7 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536625.3 | 56% | 55.9% | 1_1768del;1769_1770insT;3127C>A |
| 8 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536628.3 | 54.8% | 54.8% | 1_1854del;3214C>A |
| 9 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021155.2 | 54.8% | 54.8% | 1_1854del;3214C>A |
| 10 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021156.2 | 54.8% | 54.8% | 1_1854del;3214C>A |
| 11 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536627.3 | 54% | 53.9% | 1_1917del;3277C>A |
| 12 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XR_002957542.1 | 22.3% | 1_2020del;3380C>A;4277_10080del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2325
- ORF length:
- 2256
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaagggg cgcagggagt ctgaaagctc caggagcagc agaaggccca 121 gtggccggtc tccaaccagt actgagaagc gcatgagctt cgagtccatt tcttccctgc 181 cagaggttga gccggaccct gaggctggga gtgagcaaga ggtattttct gctgtggaag 241 ggcccagtgc cgaggagacg ccttcagaca cagaatctcc agaagtcctg gagacacagc 301 ttgatgccca ccagggcctt ctggggatgg accccccagg tgacatggtg gacttcgtgg 361 cagctgagag cactgaggac cttaaggccc tgagcagcga ggaggaagaa gaaatgggag 421 gtgccgccca ggagcctgag agccttctgc caccctctgt gctggaccag gccagcgtca 481 ttgcggagcg atttgtcagc agcttctctc ggcggagcag cgtggcacag gaggacagca 541 agtccagtgg ctttgggagc ccgcggctgg tcagccggag cagcagcgtg ctcagcctgg 601 agggcagcga gaagggcctg gcccggcatg gcagtgccac agactccctc agctgtcagc 661 tctccccaga agtggacatc agtgtggggg tggccacaga ggacagccct tctgtcaatg 721 ggatggagcc cccaagccca ggctgcccag tggagcctga ccggtcttcc tgcaagaaga 781 aggaatcagc actctccacc cgagaccggc tgttgctaga caagattaag agctattatg 841 aaaatgcaga acaccatgat gcaggcttca gcgtccgtcg ccgggagagc ctctcctaca 901 tccccaaagg actggtaaga aactccatct ccaggttcaa cagccttccc cggccagacc 961 cagagccagt acctccagtg gggagcaaga gacaggtggg ctcccggccg acttcgtggg 1021 ccctgtttga gctcccagga ccaagccagg cagtcaaagg ggacccacct cccatctcag 1081 atgctgagtt ccgcccatct tcagaaattg tgaagatctg ggagggaatg gagtcttccg 1141 gagggagccc tgggaagggg ccaggccagg gccaggccaa tggctttgac ctgcatgagc 1201 cactcttcat cctggaggag catgagctgg gagccatcac agaggagtcg gccactgcct 1261 ccccggaaag ctcctctccc actgaggggc gcagcccggc ccacctggcc cgggagctga 1321 aagagctggt gaaggagctg agcagcagta cccaggggga gctggtggcc ccactgcacc 1381 cccgcatcgt gcagctctcc cacgtaatgg acagccacgt gagcgagagc gtcaagaaca 1441 aggtctacca gctggcccgc cagtacagcc tccggatcaa gagcaacaag ccagtgatgg 1501 ccaggccacc actgcagtgg gaaaaggtgg cccctgagag ggatgggaag agccccactg 1561 tgccctgtct acaggaagag gctggagagc cattaggtgg caaaggtaag aggaagccgg 1621 tgctgtctct atttgactat gagcagctga tggcccagga gcacagccct cccaagccct 1681 cctcggctgg ggagatgtca ccacagcgtt tcttcttcaa cccgtctgct gtcagccaga 1741 ggaccacctc gcctgggggc cggccctccg cccggagccc cctcagcccc acagagacct 1801 tcagctggcc cgacgtccgt gagctctgct ccaagtatgc ctcccgcgat gaggcacgcc 1861 gagcaggggg cggccggccc cgcggcccac ccgtcaacag gagccactcg gtgccggaga 1921 acatggtaga gccacctctg tcgggcaggg tgggccgctg ccgcagcctg agcaccaaga 1981 ggggccgggg aggcggagag gctgcccaat cccctgggcc tctgccccag agcaagccgg 2041 atggaggcga gaccctgtat gtcactgcag acctcaccct ggaggacaac cggcgggtga 2101 ttgtcatgga gaagggaccc cttcccagcc ccactgcagg gctggaggag agcagtggcc 2161 agggaccaag ctcaccggtg gccctgctgg ggcaggttca ggacttccag cagtctgcag 2221 agtgccagcc gaaggaagag ggttccaggg acccggcaga cccgagccag cagggcagag 2281 tgagaaacct tagagagaag ttccaggcct tgaactctgt cggtttgcca actttcttgt 2341 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2401 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a