Construct: ORF ccsbBroad304_11842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012645.1_s304c1, BRDN0000402625
- Derived from:
- ccsbBroadEn_11842
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAU2 (27067)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11842
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164384.1 | 21.8% | 21.9% | 315_1437delinsA |
| 2 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_014393.2 | 21.8% | 21.9% | 315_1437delinsA |
| 3 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164385.1 | 20.5% | 20.6% | 0_1insATGCTTCAAATAAATCAG;297_1419delinsA |
| 4 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164381.1 | 19.4% | 19.5% | 315_1614delinsA |
| 5 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164380.2 | 17.8% | 17.5% | (many diffs) |
| 6 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347045.1 | 20.5% | 21.9% | (many diffs) |
| 7 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321109.1 | 20.1% | 21.4% | (many diffs) |
| 8 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347044.1 | 19% | 19.5% | (many diffs) |
| 9 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238390.2 | 18.6% | 19.1% | (many diffs) |
| 10 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001111272.1 | 18.2% | 19.5% | (many diffs) |
| 11 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495532.3 | 18.2% | 19.5% | (many diffs) |
| 12 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238389.1 | 18.2% | 19.5% | (many diffs) |
| 13 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238388.2 | 17.7% | 18.2% | (many diffs) |
| 14 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_025303.3 | 17% | 17.5% | (many diffs) |
| 15 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495528.3 | 17% | 17.5% | (many diffs) |
| 16 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238387.1 | 17% | 17.5% | (many diffs) |
| 17 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321102.1 | 17% | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tcaaataaat cagatgttct cagtgcagct gagtcttggt gagcagacat 121 gggaatccga aggcagcagt ataaagaagg ctcagcaggc tgttgccaat aaagctttga 181 ctgaatctac gcttcccaaa ccagttcaga agccacccaa aagtaatgtt aacaataacc 241 caggcagtat aactccaact gtggaactga atgggcttgc tatgaaaagg ggagagcctg 301 ccatctacag gccattagat ccaaagccat tcccaaatta tagagctaat tacaactttc 361 ggggcatgta caatcagaga tgcccaactt tcttgtacaa agtggttggt aagcctatcc 421 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 481 ttaagtcgac aatcaacctc tggatta