Construct: ORF ccsbBroad304_11848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003665.1_s304c1, BRDN0000462097
- Derived from:
- ccsbBroadEn_11848
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND2A (27147)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11848
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27147 | DENND2A | DENN domain containing 2A | NM_001318053.2 | 99.9% | 99.8% | 467C>A |
| 2 | human | 27147 | DENND2A | DENN domain containing 2A | XM_005249979.4 | 92.4% | 91.4% | (many diffs) |
| 3 | human | 27147 | DENND2A | DENN domain containing 2A | NM_001318052.1 | 78% | 76.1% | (many diffs) |
| 4 | human | 27147 | DENND2A | DENN domain containing 2A | NM_001362678.1 | 78% | 76.1% | (many diffs) |
| 5 | human | 27147 | DENND2A | DENN domain containing 2A | NM_015689.5 | 78% | 76.1% | (many diffs) |
| 6 | human | 27147 | DENND2A | DENN domain containing 2A | XM_011516053.2 | 78% | 76.1% | (many diffs) |
| 7 | human | 27147 | DENND2A | DENN domain containing 2A | XM_017011989.1 | 78% | 76.1% | (many diffs) |
| 8 | human | 27147 | DENND2A | DENN domain containing 2A | XM_011516054.2 | 77.9% | 76% | (many diffs) |
| 9 | human | 27147 | DENND2A | DENN domain containing 2A | NR_134477.1 | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2454
- ORF length:
- 2385
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatatgttc agcttggata tgatcatcag tgacccagct gcagaagcca 121 gcagggctgg gaagaagcag ctcagaggtg ttcagaaccc ttgcccatct gccagagcca 181 gaccccggca caagtccctc aacataaagg acaagatatc agaatgggaa gggaagaaag 241 aggtgcccac tcctgcaccc agcaggagag cagacggaca ggaggattat ctgccgtcct 301 ctacggtgga gaggaggagt agtgatgggg tgagaactca ggtcacagag gctaagaatg 361 gaatgaggcc aggaacagag agcacagaga aggagaggaa taaaggagca gtgaacgtcg 421 ggggacagga cccagagccg gggcaagacc taagccagcc agaacgggaa gtggatccta 481 gctggggccg aggccgagag ccaagacttg gcaagctacg ctttcagaac gatcacctct 541 ccgtgctgaa gcaggtcaag aaactcgagc aggctttgaa ggatgggtcg gcagggctgg 601 atccccagtt accagggact tgttactccc cacactgccc tcctgacaag gcagaggcag 661 ggtccaccct tcctgagaac ctgggaggcg ggagtggctc agaagtcagc cagagggtcc 721 acccctcgga cctggaaggc agggagccca cccctgagct tgtggaggac aggaaaggtt 781 catgcagaag gccctgggac cggagccttg agaacgtgta taggggctcg gagggttccc 841 ccacaaagcc cttcatcaac cctctgccaa aaccccggag aacgttcaaa catgccggag 901 aaggggacaa agatgggaag cctggcatcg gcttcaggaa agagaaaaga aatctgcctc 961 ctctgccctc tctacctccc ccgcctctgc cctcctctcc cccaccttcc tctgtgaaca 1021 gaagactgtg gaccgggaga cagaaatcca gtgcagacca cagaaagtcc tatgagtttg 1081 aagatttact gcagtcttcc tctgagagca gcagggtgga ctggtacgcg cagactaagc 1141 tggggctgac acgcacttta tcggaggaga acgtctatga agacattcta gatccgccaa 1201 tgaaggagaa cccttatgag gacatcgagt tacatggtcg ctgcctggga aagaagtgtg 1261 tcttgaattt tcctgcttct cccacctctt ccatccctga cacactcacc aagcagtcat 1321 tgtccaaacc tgcttttttc cgacaaaatt cagagaggag gaacttcaag ctgctggaca 1381 ctaggaagct gagtcgggat ggaactgggt ccccttccaa aatcagccct ccctccactc 1441 ccagcagccc tgatgacatt ttctttaacc ttggagaccc acagaacggc aggaagaaga 1501 gaaagatacc caagctggtg ttgcgaatca acgccattta tgaggtccgg agaggaaaga 1561 aacgggtgaa gaggctgtcc cagtcaatgg agagcaactc aggaaaagtg acagatgaga 1621 acagtgagtc tgacagtgac acagaggaga agctgaaagc tcacagccag cgcctggtca 1681 acgtgaagtc ccggctgaag caggcgcctc ggtacccatc acttgcccgg gaactcatcg 1741 agtaccagga gaggcagctc ttcgagtact ttgtggttgt gtctttgcac aagaagcagg 1801 ccggggctgc ctacgtgcca gaactcaccc aacagttccc tctgaagttg gaaaggtctt 1861 tcaagttcat gagagaagct gaggaccaac tgaaggccat tccccagttc tgttttcccg 1921 atgccaagga ttgggttcct gtccagcagt tcaccagtga aacattctca tttgtcttaa 1981 ctggagaaga tgggagcaga aggttcggtt actgccgaag actgctgcct ggaggcaaag 2041 ggaagcgcct tcctgaagtt tactgcattg tgagccgcct gggatgcttc agcctctttt 2101 caaggatctt ggatgaggtg gaaaaaagac gaggcatctc tcctgccctg gttcagccac 2161 tcatgagaag tgtcatggaa gcccctttcc cagccctggg caaaaccatc cttgtcaaga 2221 acttcctgcc aggttcagga actgaggtga tcgaactgtg ccgcccgctg gactcccggc 2281 tcgagcacgt ggactttgag tctctcttct cctccctcag cgtccgccac ctggtctgtg 2341 tgtttgcctc cctgcttctg gagaggaggg tcatcttcat tgcagacaag ctcaggtacc 2401 cgccttggct acttctgcta aagcgtttga atgacagtcg ctcttggacc ctcttgccaa 2461 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2521 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta