Construct: ORF ccsbBroad304_11848

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003665.1_s304c1, BRDN0000462097
Derived from:
ccsbBroadEn_11848
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DENND2A (27147)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11848
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27147 DENND2A DENN domain containing 2A NM_001318053.2 99.9% 99.8% 467C>A
2 human 27147 DENND2A DENN domain containing 2A XM_005249979.4 92.4% 91.4% (many diffs)
3 human 27147 DENND2A DENN domain containing 2A NM_001318052.1 78% 76.1% (many diffs)
4 human 27147 DENND2A DENN domain containing 2A NM_001362678.1 78% 76.1% (many diffs)
5 human 27147 DENND2A DENN domain containing 2A NM_015689.5 78% 76.1% (many diffs)
6 human 27147 DENND2A DENN domain containing 2A XM_011516053.2 78% 76.1% (many diffs)
7 human 27147 DENND2A DENN domain containing 2A XM_017011989.1 78% 76.1% (many diffs)
8 human 27147 DENND2A DENN domain containing 2A XM_011516054.2 77.9% 76% (many diffs)
9 human 27147 DENND2A DENN domain containing 2A NR_134477.1 68.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2454
ORF length:
2385
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggatatgttc agcttggata tgatcatcag tgacccagct gcagaagcca
121gcagggctgg gaagaagcag ctcagaggtg ttcagaaccc ttgcccatct gccagagcca
181gaccccggca caagtccctc aacataaagg acaagatatc agaatgggaa gggaagaaag
241aggtgcccac tcctgcaccc agcaggagag cagacggaca ggaggattat ctgccgtcct
301ctacggtgga gaggaggagt agtgatgggg tgagaactca ggtcacagag gctaagaatg
361gaatgaggcc aggaacagag agcacagaga aggagaggaa taaaggagca gtgaacgtcg
421ggggacagga cccagagccg gggcaagacc taagccagcc agaacgggaa gtggatccta
481gctggggccg aggccgagag ccaagacttg gcaagctacg ctttcagaac gatcacctct
541ccgtgctgaa gcaggtcaag aaactcgagc aggctttgaa ggatgggtcg gcagggctgg
601atccccagtt accagggact tgttactccc cacactgccc tcctgacaag gcagaggcag
661ggtccaccct tcctgagaac ctgggaggcg ggagtggctc agaagtcagc cagagggtcc
721acccctcgga cctggaaggc agggagccca cccctgagct tgtggaggac aggaaaggtt
781catgcagaag gccctgggac cggagccttg agaacgtgta taggggctcg gagggttccc
841ccacaaagcc cttcatcaac cctctgccaa aaccccggag aacgttcaaa catgccggag
901aaggggacaa agatgggaag cctggcatcg gcttcaggaa agagaaaaga aatctgcctc
961ctctgccctc tctacctccc ccgcctctgc cctcctctcc cccaccttcc tctgtgaaca
1021gaagactgtg gaccgggaga cagaaatcca gtgcagacca cagaaagtcc tatgagtttg
1081aagatttact gcagtcttcc tctgagagca gcagggtgga ctggtacgcg cagactaagc
1141tggggctgac acgcacttta tcggaggaga acgtctatga agacattcta gatccgccaa
1201tgaaggagaa cccttatgag gacatcgagt tacatggtcg ctgcctggga aagaagtgtg
1261tcttgaattt tcctgcttct cccacctctt ccatccctga cacactcacc aagcagtcat
1321tgtccaaacc tgcttttttc cgacaaaatt cagagaggag gaacttcaag ctgctggaca
1381ctaggaagct gagtcgggat ggaactgggt ccccttccaa aatcagccct ccctccactc
1441ccagcagccc tgatgacatt ttctttaacc ttggagaccc acagaacggc aggaagaaga
1501gaaagatacc caagctggtg ttgcgaatca acgccattta tgaggtccgg agaggaaaga
1561aacgggtgaa gaggctgtcc cagtcaatgg agagcaactc aggaaaagtg acagatgaga
1621acagtgagtc tgacagtgac acagaggaga agctgaaagc tcacagccag cgcctggtca
1681acgtgaagtc ccggctgaag caggcgcctc ggtacccatc acttgcccgg gaactcatcg
1741agtaccagga gaggcagctc ttcgagtact ttgtggttgt gtctttgcac aagaagcagg
1801ccggggctgc ctacgtgcca gaactcaccc aacagttccc tctgaagttg gaaaggtctt
1861tcaagttcat gagagaagct gaggaccaac tgaaggccat tccccagttc tgttttcccg
1921atgccaagga ttgggttcct gtccagcagt tcaccagtga aacattctca tttgtcttaa
1981ctggagaaga tgggagcaga aggttcggtt actgccgaag actgctgcct ggaggcaaag
2041ggaagcgcct tcctgaagtt tactgcattg tgagccgcct gggatgcttc agcctctttt
2101caaggatctt ggatgaggtg gaaaaaagac gaggcatctc tcctgccctg gttcagccac
2161tcatgagaag tgtcatggaa gcccctttcc cagccctggg caaaaccatc cttgtcaaga
2221acttcctgcc aggttcagga actgaggtga tcgaactgtg ccgcccgctg gactcccggc
2281tcgagcacgt ggactttgag tctctcttct cctccctcag cgtccgccac ctggtctgtg
2341tgtttgcctc cctgcttctg gagaggaggg tcatcttcat tgcagacaag ctcaggtacc
2401cgccttggct acttctgcta aagcgtttga atgacagtcg ctcttggacc ctcttgccaa
2461ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2521cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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