Construct: ORF ccsbBroad304_11924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003633.1_s304c1, BRDN0000399626
- Derived from:
- ccsbBroadEn_11924
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEF6 (50619)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11924
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50619 | DEF6 | DEF6 guanine nucleotide exc... | NM_022047.4 | 53.1% | 53% | 860A>C;1009_1893del |
| 2 | mouse | 23853 | Def6 | differentially expressed in... | NM_027185.3 | 47.4% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgcgcaag gaactgctca agtccatctg gtacgccttt accgcgctgg 121 acgtggagaa gagtggcaaa gtctccaagt cccagctcaa ggtgctgtcc cacaacctgt 181 acacggtcct gcacatcccc catgaccccg tggccctgga ggaacacttc cgagatgatg 241 atgacggccc tgtgtccagc cagggataca tgccctacct caacaagtac atcctggaca 301 aggtggagga gggggctttt gttaaagagc actttgatga gctgtgctgg acgctgacgg 361 ccaagaagaa ctatcgggca gatagcaacg ggaacagtat gctctccaat caggatgcct 421 tccgcctctg gtgcctcttc aacttcctgt ctgaggacaa gtaccctctg atcatggttc 481 ctgatgaggt ggaatacctg ctgaaaaagg tactcagcag catgagcttg gaggtgagct 541 tgggtgagct ggaggagctt ctggcccagg aggcccaggt ggcccagacc accggggggc 601 tcagcgtctg gcagttcctg gagctcttca attcgggccg ctgcctgcgg ggcgtgggcc 661 gggacaccct cagcatggcc atccacgagg tctaccagga gctcatccaa gatgtcctga 721 agcagggcta cctgtggaag cgagggcacc tgagaaggaa ctgggccgaa cgctggttcc 781 agctgcagcc cagctgcctc tgctactttg ggagtgaaga gtgcaaagag aaaaggggca 841 ttatcccgct ggatgcacac tgctgcgtgg aggtgctgcc agaccgcgac ggaaagcgct 901 gcatgttctg tgtgaagaca gccacccgca cgtatgagat gagcgcctca gacacgcgcc 961 agcgccagga gtggacagct gccatccaga tggcgatccg gctgcaggcc gaggggaaga 1021 cgtccctaca caaggacctg aagcagaaac ggcgcgagca gcgggagcag cggtacccaa 1081 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1141 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta