Construct: ORF ccsbBroad304_11932

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014616.1_s304c1, BRDN0000408992
Derived from:
ccsbBroadEn_11932
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VILL (50853)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11932
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50853 VILL villin like NM_001370264.1 97.9% 97.9% 274_315del
2 human 50853 VILL villin like NM_001370265.1 96% 93.1% (many diffs)
3 human 50853 VILL villin like NM_015873.3 78.5% 78.5% 1_510del;784_825del
4 human 50853 VILL villin like NR_163266.1 74.3% (many diffs)
5 human 50853 VILL villin like NR_163267.1 72.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2082
ORF length:
2016
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat gattcagtgg aatgggccca agaccagcat ttctgagaag gctcgggggc
121tggctttgac ctacagcctc cgggacaggg aacgtggtgg tggtcgtgca cagattggtg
181tggtggatga tgaggccaaa gccccggacc tcatgcagat catggaggct gtgctgggcc
241gcagggtggg cagcctgcgt gccgccacgc ccagcaagga tatcaaccag ctgcagaagg
301ccaatgttcg cctgtaccat gtctatgaga agggcaaaga cctgctgcag gaggaggact
361tctacatcct ggaccagggt ggcttcaaga tctatgtgtg gcagggacgc atgtctagcc
421tccaggagag aaaggctgcc ttcagccggg ctgtgggctt catccaggcc aagggctacc
481cgacctacac caacgtggag gtggtgaacg acggcgccga gtcggccgcg ttcaagcagc
541tcttccggac ttggtctgag aagcggcgca ggaaccagaa gctcggcggg agggataaat
601cgattcatgt aaagctggac gtgggcaagc tgcacaccca gcctaagtta gcggcccagc
661tcaggatggt ggacgacggc tctgggaagg tggaggtgtg gtgcatccag gacttacaca
721ggcagcccgt ggaccccaag cgtcatggac agctgtgtgc aggcaactgc taccttgtgc
781tctacacata ccagaggctg ggccgtgtcc agtacatcct gtacctatgg cagggccacc
841aggccactgc ggatgagatt gaggccctga acagcaacgc tgaggaacta gatgtcatgt
901atggtggcgt cctagtacag gagcatgtga ccatgggcag cgagcccccc cacttcctcg
961ccatcttcca gggccagctg gtgatcttcc aggagagagc tgggcaccat ggaaaggggc
1021agtcagcatc caccacaagg cttttccaag tgcaaggcac tgacagccac aacaccagga
1081ccatggaggt gccagcccgt gcctcatccc tcaactccag tgacatcttc ttgctggtca
1141cagccagcgt ctgctacctc tggtttggga agggctgtaa tggtgatcag cgtgagatgg
1201cacgggtggt ggtcactgtc atttccagga agaatgagga aacggtgctg gagggtcagg
1261agcctcccca cttctgggag gccctgggag gccgggcccc ctaccccagc aacaagaggc
1321tccctgagga ggtccccagc ttccagccac gactgtttga gtgctccagc cacatgggct
1381gcctggtcct cgcagaagtg gggttcttca gccaggagga cctggacaag tatgacatca
1441tgttactgga cacctggcag gagatcttcc tgtggcttgg ggaagctgca agtgagtgga
1501aggaggcggt ggcctggggc caggagtacc tgaagactca cccagcaggg aggagcccgg
1561ccacacccat cgtgctggtc aagcagggcc atgagcctcc caccttcatt ggatggttct
1621tcacttggga cccctacaag tggactagcc acccgtccca caaggaagtg gtggatggca
1681gcccggcagc agcatcaacc atctctgaga taacagcaga agtcaacaac ttgcggctat
1741ccagatggcc gggcaatggc agggcaggtg ccgtggccct gcaggccctc aagggctccc
1801aggacagctc agagaatgat ctggtgcgaa gccccaagtc ggctggcagc agaaccagca
1861gctccgtcag cagcaccagc gccacgatca acgggggcct gcgccgggaa caactgatgc
1921accaggctgt tgaggacctg ccagagggcg tggaccctgc ccgcagggag ttctatctct
1981cagactctga cttccaagat atctttggga aatccaagga ggaattctac agcatggcca
2041cgtggaggca gcggcaggag aaaaagcagc tgggcttctt ctgcccaact ttcttgtaca
2101aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2161ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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