Transcript: Human NM_015873.3

Homo sapiens villin like (VILL), mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
VILL (50853)
Length:
2787
CDS:
87..2657

Additional Resources:

NCBI RefSeq record:
NM_015873.3
NBCI Gene record:
VILL (50853)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015873.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423322 CATCAACCATCTCTGAGATAA pLKO_005 2266 CDS 100% 13.200 18.480 N VILL n/a
2 TRCN0000092000 GACAAGTATGACATCATGTTA pLKO.1 1998 CDS 100% 5.625 7.875 N Vill n/a
3 TRCN0000113883 CGCTGAGGAACTAGATGTCAT pLKO.1 1451 CDS 100% 4.950 3.960 N VILL n/a
4 TRCN0000417969 AGCGACTGCTGCACATCAAAG pLKO_005 490 CDS 100% 10.800 7.560 N VILL n/a
5 TRCN0000113882 CCTGGACAAGTATGACATCAT pLKO.1 1994 CDS 100% 4.950 3.465 N VILL n/a
6 TRCN0000113884 GCTACCTTGTGCTCTACACAT pLKO.1 1342 CDS 100% 4.950 3.465 N VILL n/a
7 TRCN0000113885 CAGAGAATGATCTGGTGCGAA pLKO.1 2383 CDS 100% 2.640 1.848 N VILL n/a
8 TRCN0000113881 AGCCCTCTCGACTGCCCCTAT pLKO.1 2663 3UTR 100% 0.000 0.000 N VILL n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015873.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11933 pDONR223 100% 80.1% 80.1% None 1_510del;2382G>T n/a
2 ccsbBroad304_11933 pLX_304 0% 80.1% 80.1% V5 1_510del;2382G>T n/a
3 TRCN0000476455 GCGATAACAAACATGGATTTCCAA pLX_317 13.6% 80.1% 80.1% V5 1_510del;2382G>T n/a
4 ccsbBroadEn_11932 pDONR223 100% 78.5% 78.5% None 1_510del;784_825del n/a
5 ccsbBroad304_11932 pLX_304 0% 78.5% 78.5% V5 1_510del;784_825del n/a
6 TRCN0000479437 TTCCAAGGGCTAGGCTGATAGGCC pLX_317 19% 78.5% 78.5% V5 1_510del;784_825del n/a
Download CSV