Transcript: Human NR_163266.1

Homo sapiens villin like (VILL), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-05-05
Taxon:
Homo sapiens (human)
Gene:
VILL (50853)
Length:
2711
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_163266.1
NBCI Gene record:
VILL (50853)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_163266.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423322 CATCAACCATCTCTGAGATAA pLKO_005 2168 3UTR 100% 13.200 18.480 N VILL n/a
2 TRCN0000092000 GACAAGTATGACATCATGTTA pLKO.1 1900 3UTR 100% 5.625 7.875 N Vill n/a
3 TRCN0000113883 CGCTGAGGAACTAGATGTCAT pLKO.1 1353 3UTR 100% 4.950 3.960 N VILL n/a
4 TRCN0000417969 AGCGACTGCTGCACATCAAAG pLKO_005 392 3UTR 100% 10.800 7.560 N VILL n/a
5 TRCN0000113882 CCTGGACAAGTATGACATCAT pLKO.1 1896 3UTR 100% 4.950 3.465 N VILL n/a
6 TRCN0000113884 GCTACCTTGTGCTCTACACAT pLKO.1 1244 3UTR 100% 4.950 3.465 N VILL n/a
7 TRCN0000113885 CAGAGAATGATCTGGTGCGAA pLKO.1 2307 3UTR 100% 2.640 1.848 N VILL n/a
8 TRCN0000113881 AGCCCTCTCGACTGCCCCTAT pLKO.1 2587 3UTR 100% 0.000 0.000 N VILL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_163266.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11933 pDONR223 100% 75.8% None (many diffs) n/a
2 ccsbBroad304_11933 pLX_304 0% 75.8% V5 (many diffs) n/a
3 TRCN0000476455 GCGATAACAAACATGGATTTCCAA pLX_317 13.6% 75.8% V5 (many diffs) n/a
4 ccsbBroadEn_11932 pDONR223 100% 74.3% None (many diffs) n/a
5 ccsbBroad304_11932 pLX_304 0% 74.3% V5 (many diffs) n/a
6 TRCN0000479437 TTCCAAGGGCTAGGCTGATAGGCC pLX_317 19% 74.3% V5 (many diffs) n/a
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